Shibata Yasuhiro, Kashiwagi Bunzo, Ono Yoshihiro, Fukabori Yoshitatsu, Suzuki Kazuhiro, Honma Seijiro, Yamanaka Hidetoshi
Department of Urology, Gunma University School of Medicine, Maebashi, Gunma, Japan.
Urol Res. 2004 Feb;32(1):44-8. doi: 10.1007/s00240-003-0375-5. Epub 2003 Oct 8.
To evaluate the accuracy and reliability of laser speckle blood flowmetry in the measurement of prostate blood flow, we performed a comparative study with the ordinary intra-arterial radioactive microsphere injection method using the well-known castrate-rat model. Adult male Wistar strain rats were used for the study. The rats were either only castrated or subcutaneous testosterone replenishment was followed subcutaneously 6 h after castration. The prostate blood flow was measured at different time courses after castration. The effect of a single androgen replenishment on prostate blood flow was also evaluated. The measurement of prostate blood flow was performed by both the radioactive microsphere injection method and laser speckle blood flowmetry, and then the outcomes were compared. Rapid reduction in prostate blood flow to 30% of the normal level was observed 24 h after castration in the measurements with the microsphere injection technique. The prostate blood flow decreased gradually after 24 h post-castration to 23 and 21% of the normal level at 48 and 72 h after castration, respectively. The laser speckle blood flowmetry also detected the decrease in prostate blood flow well, but in a more gradual manner. The prostate blood flow was 70, 52 and 35% of the normal level at 24, 48 and 72 h after castration, respectively. The effect of a single administration of testosterone to castrated rats had recovered the prostate blood flow to 74 and 98% of the normal level by measurement with the microsphere injection technique and laser speckle blood flowmetry, respectively. The different outcome in blood flow rate change between the methods can be explained according to their different mechanism of measurement, thus suggesting the capillary vessels are the early and most responsive portion for hormonal manipulation. In conclusion, the laser speckle blood flowmetry is a convenient and reliable method for evaluating prostate blood flow, especially when the organ is required for other biological and molecular assays, since the method does not require the excision of the organ for the measurement.
为评估激光散斑血流仪在测量前列腺血流方面的准确性和可靠性，我们使用著名的去势大鼠模型，与普通动脉内放射性微球注射法进行了一项对比研究。成年雄性Wistar品系大鼠用于该研究。大鼠要么仅接受去势手术，要么在去势后6小时皮下补充睾酮。在去势后的不同时间点测量前列腺血流。还评估了单次雄激素补充对前列腺血流的影响。通过放射性微球注射法和激光散斑血流仪测量前列腺血流，然后比较结果。在微球注射技术测量中，去势后24小时观察到前列腺血流迅速降至正常水平的30%。去势后24小时后，前列腺血流逐渐下降，在去势后48小时和72小时分别降至正常水平的23%和21%。激光散斑血流仪也能很好地检测到前列腺血流的下降，但下降方式更为渐进。去势后24小时、48小时和72小时，前列腺血流分别为正常水平的70%、52%和35%。对去势大鼠单次注射睾酮的效果，通过微球注射技术测量，前列腺血流恢复到正常水平的74%，通过激光散斑血流仪测量则恢复到正常水平的98%。两种方法在血流速率变化上的不同结果可根据其不同的测量机制来解释，这表明毛细血管是激素调控的早期且最敏感的部分。总之，激光散斑血流仪是评估前列腺血流的一种便捷可靠的方法，特别是当该器官需要用于其他生物学和分子检测时，因为该方法不需要切除器官进行测量。
