大鼠脑皮质切片将L-亮氨酸中的15N掺入异亮氨酸的过程。
Incorporation of 15N from L-leucine into isoleucine by rat brain cerebral cortex slices.
作者信息
Gheorvassaki E, Papadakis D, Alikaridis F, Kephalas T, Larsen C, Palaiologos G
机构信息
Laboratory of Biological Chemistry, School of Medicine, University of Athens, Greece.
出版信息
J Neurosci Res. 1992 Oct;33(2):306-9. doi: 10.1002/jnr.490330214.
The fate of leucine nitrogen in the central nervous system was investigated by incubating rat cerebral cortex slices in the presence of 0.5 mM each of L-[15N]-leucine, L-isoleucine, and L-valine. Analysis of the slices and incubation media for free amino acids by gas chromatography-mass spectrometry revealed that the 15N from leucine is incorporated into isoleucine only. No 15N was detected in valine or any other amino acid. These results suggest that leucine, valine, and their corresponding aminotransferases may be compartmentalized in brain cerebral cortex.
通过在含有0.5 mM的L-[¹⁵N]-亮氨酸、L-异亮氨酸和L-缬氨酸的条件下孵育大鼠大脑皮层切片，研究了中枢神经系统中亮氨酸氮的命运。通过气相色谱-质谱联用仪对切片和孵育培养基中的游离氨基酸进行分析，结果显示，来自亮氨酸的¹⁵N仅掺入异亮氨酸中。在缬氨酸或任何其他氨基酸中均未检测到¹⁵N。这些结果表明，亮氨酸、缬氨酸及其相应的转氨酶可能在大脑皮层中被分隔开来。
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