Camorlinga-Ponce Margarita, Aviles-Jimenez Francisco, Cabrera Lourdes, Hernández-Pando Rogelio, Muñoz Onofre, Soza Jorge, Torres Javier
Unidad de Investigación Médica en Enfermedades Infecciosas, Instituto Nacional de la Nutrición Salvador Subirán, Mexico.
Helicobacter. 2003;8(5):554-60. doi: 10.1046/j.1523-5378.2003.00176.x.
Few reports exist on inflammation and interleukin (IL)-8 response in H. pylori-infected children. The aim of this study was to determine the intensity of inflammation, density of colonization and magnitude of IL-8 response in children with and without H. pylori infection.
We studied 45 children with dyspeptic symptoms, 21 infected with H. pylori and 24 without infection. Antrum and corpus gastric biopsies were obtained and studied for H. pylori infection with an immunofluorescence technique and for IL-8 with an immunohistochemical assay. Biopsy specimens were stained with hematoxilin and eosin and gastritis was graded according to the Sydney system. The magnitudes of the IL-8 response and H. pylori colonization were estimated microscopically with image analyzer software.
In H. pylori-infected children, mild mono-nuclear cell infiltration was found in 50%, and no neutrophils in 40% of cases. In the antrum but not in the corpus, the intensity of colonization correlated with neutrophil and mononuclear cell infiltration. The IL-8 response was significantly higher in the antrum (p <.05) and corpus (p <.02) of infected children, and was localized mainly in the surface and crypts of the epithelium. No correlation was found between the magnitude of the IL-8 response and the infiltration of either neutrophil or mononuclear cells.
In H. pylori-infected children, poor mononuclear and neutrophil infiltration was observed. Infection was associated with a higher IL-8 response by gastric epithelial cells. The density of colonization but not the IL-8 response correlated with neutrophil cell infiltration.
关于幽门螺杆菌感染儿童的炎症和白细胞介素（IL）-8反应的报道较少。本研究的目的是确定幽门螺杆菌感染和未感染儿童的炎症强度、定植密度和IL-8反应程度。
我们研究了45名有消化不良症状的儿童，其中21名感染幽门螺杆菌，24名未感染。获取胃窦和胃体活检组织，采用免疫荧光技术检测幽门螺杆菌感染情况，采用免疫组织化学分析法检测IL-8。活检标本用苏木精和伊红染色，根据悉尼系统对胃炎进行分级。用图像分析软件在显微镜下估计IL-8反应程度和幽门螺杆菌定植情况。
在幽门螺杆菌感染儿童中，50%的病例发现轻度单核细胞浸润，40%的病例未发现中性粒细胞。在胃窦而非胃体中，定植强度与中性粒细胞和单核细胞浸润相关。感染儿童胃窦（p<.05）和胃体（p<.02）的IL-8反应明显更高，且主要定位于上皮表面和隐窝。未发现IL-8反应程度与中性粒细胞或单核细胞浸润之间存在相关性。
在幽门螺杆菌感染儿童中，观察到单核细胞和中性粒细胞浸润较少。感染与胃上皮细胞更高的IL-8反应相关。定植密度与中性粒细胞浸润相关，而与IL-8反应无关。
