Tisserat B, Murashige T
In Vitro. 1977 Nov;13(11):799-805. doi: 10.1007/BF02627860.
A factor that represses asexual embryogenesis has been observed in the Rutaceae, with particularly high concentrations in the naturally monoembryonic cultivars. This investigation was an initial step towards identifying the factor. Citrus reticulata Blanco Ponkan mandarin nucellus explants and Daucus carota L. 'Queen Anne's Lace' callus were employed to examine effects of known plant growth regulators and to determine possible identity of one or more of them with the repressive factor. The chalazal halves of ovules of C. media L. 'Citron of Commerce' were used as control repressor source. Embryo initiation and growth of both test tissues were depressed markedly by 2,4-D, abscisic acid and ethephon. Slight inhibitions were obtained with IAA, kinetin and gibberellic acid. Recovery from the repressor did not occur readily in Citrus nucellus following recultures in citron-ovule-free medium; carrot callus resumed normal embryogenesis immediately upon transfer to suppressor-free medium. The repression by natural sources apparently involved the combined action of some or all natural hormones that are generically related to the above.
在芸香科中已观察到一种抑制无融合生殖胚胎发生的因子，在天然单胚品种中其浓度特别高。本研究是鉴定该因子的第一步。采用柑桔属的红桔“椪柑”珠心外植体和胡萝卜属的“安妮女王的蕾丝”胡萝卜愈伤组织来研究已知植物生长调节剂的作用，并确定其中一种或多种与抑制因子的可能一致性。用甜橙属的“商业香橼”胚珠合点端的一半作为对照抑制源。2,4 - D、脱落酸和乙烯利显著抑制了两种试验组织的胚胎起始和生长。吲哚乙酸、激动素和赤霉素有轻微抑制作用。在无香橼胚珠培养基上继代培养后，柑桔属珠心组织不易从抑制因子中恢复；胡萝卜愈伤组织转移到无抑制因子培养基后立即恢复正常胚胎发生。天然来源的抑制作用显然涉及与上述物质一般相关的某些或所有天然激素的联合作用。
