Department of Plant Sciences, University of California, Riverside, California 92521.
Plant Physiol. 1977 Sep;60(3):437-9. doi: 10.1104/pp.60.3.437.
Asexual embryogenesis in Daucus carota L. ;Queen Anne's Lace' callus was suppressed by Ethephon, ethylene, and 2,4-dichlorophenoxyacetic acid (2,4-D). The Ethephon effect could be attributed to volatile and nonvolatile substances. The volatile component was probably entirely ethylene. Ethylene was liberated in the cultures in direct proportion to Ethephon added to the medium. Autoclaving of Ethephon caused a substantial decrease of measurable ethylene. Continuous exposure of callus to 5 mul/l ethylene depressed somatic cell embryogenesis, but not markedly. Depression of embryogenesis by 2,4-D was unrelated to ethylene evolution.
胡萝卜的无融合生殖；‘野胡萝卜’愈伤组织的形成被乙烯磷、乙烯和 2，4-二氯苯氧乙酸（2，4-D）所抑制。乙烯磷的作用可能归因于挥发性和非挥发性物质。挥发性成分很可能完全是乙烯。乙烯在培养基中添加到与乙烯磷直接成比例的培养物中释放出来。乙烯磷的高压灭菌导致可测量的乙烯大量减少。将愈伤组织连续暴露于 5 μl/l 的乙烯中会抑制体细胞胚胎发生，但不显著。2，4-D 对胚胎发生的抑制与乙烯的产生无关。
