Hutchinson M J, KrishnaRaj S, Saxena P K
Department of Horticultural Science, University of Guelph, N1G 2W1, Guelph, Ontario, Canada.
Plant Cell Rep. 1997 Mar;16(6):435-438. doi: 10.1007/BF01146789.
Somatic embryogenesis in geranium (Pelargonium xhortorum Bailey cv 'Scarlet Orbit Improved') can be achieved by incubating hypocotyl explants on MS medium supplemented with thidiazuron (TDZ; 10 μM for 3 days followed by subculture on medium devoid of any plant growth regulators. The presence of gibberellins (GAs) during both the induction and expression phases of embryogenesis was significantly detrimental to somatic embryo formation on the hypocotyl explants. The addition of the GA-synthesis inhibitors paclobutrazol, uniconazole or ancymidol during the period of growth and differentiation of somatic embryos increased the number of somatic embryos formed on each explant. However, paclobutrazol added during the period of induction had no significant influence on somatic embryo formation. Results suggest that both exogenously supplied as well as endogenous GAs play a role, albeit a negative one, on somatic embryogenesis of geranium.
天竺葵（Pelargonium xhortorum Bailey cv 'Scarlet Orbit Improved'）的体细胞胚胎发生可以通过将下胚轴外植体接种在添加了噻二唑素（TDZ；10 μM处理3天，随后转接至不含任何植物生长调节剂的培养基上）的MS培养基上来实现。在胚胎发生的诱导期和表达期，赤霉素（GAs）的存在对下胚轴外植体上体细胞胚胎的形成有显著的不利影响。在体细胞胚胎生长和分化阶段添加GA合成抑制剂多效唑、烯效唑或嘧啶醇，可增加每个外植体上形成的体细胞胚胎数量。然而，在诱导期添加多效唑对体细胞胚胎的形成没有显著影响。结果表明，无论是外源供应的还是内源的GA，尽管对天竺葵的体细胞胚胎发生起负面作用，但都发挥了作用。
