Doughty M J
School of Optometry, University of Waterloo, Ontario, Canada.
Optom Vis Sci. 1992 Nov;69(11):846-57. doi: 10.1097/00006324-199211000-00003.
Whole corneas from 2- to 2.5-kg albino rabbits were perfused in vitro at 35 to 36 degrees C with a commercial balanced salt solution (BSS) that lacks bicarbonate and glucose. The corneas swelled at an average rate of 45 +/- 11 microns/h. Evaluation of the corneal endothelium by scanning electron microscopy revealed no obvious cytotoxic effect beyond the occasional small area affected by air bubble damage. Morphometric analysis of the central corneal endothelium revealed no significant differences between the endothelial mosaic of perfused corneas, when compared to freshly fixed corneas, in terms of percentage 6-sided cells (pleomorphism) and variance in cell areas (polymegethism). In this example, therefore, a corneal swelling induced by a bicarbonate and glucose-free BSS does not appear to be due to either obvious endothelial cytotoxic effects or significant morphological alteration of the endothelial mosaic.
将体重2至2.5千克的白化兔的全角膜在体外35至36摄氏度下，用一种不含碳酸氢盐和葡萄糖的商用平衡盐溶液（BSS）进行灌注。角膜以平均45±11微米/小时的速率肿胀。通过扫描电子显微镜对角膜内皮进行评估，结果显示除了偶尔受气泡损伤影响的小区域外，没有明显的细胞毒性作用。对中央角膜内皮进行形态计量分析发现，与新鲜固定的角膜相比，灌注角膜的内皮镶嵌在六边形细胞百分比（多形性）和细胞面积差异（大小不均一性）方面没有显著差异。因此，在这个例子中，由不含碳酸氢盐和葡萄糖的BSS引起的角膜肿胀似乎既不是由于明显的内皮细胞毒性作用，也不是由于内皮镶嵌的显著形态改变。
