McDermott M, Snyder R, Slack J, Holley G, Edelhauser H
Department of Ophthalmology, Kresge Eye Institute, Wayne State University, Detroit, Michigan 48201.
Cornea. 1991 Sep;10(5):402-7. doi: 10.1097/00003226-199109000-00008.
Either a simple balanced salt solution (BSS) or a bicarbonate-buffered, glutathione-containing commercial irrigant (BSS Plus, Alcon Laboratories, Fort Worth, Texas, U.S.A.) may be used during the vitrectomy portion of a corneal transplant procedure. To simulate the conditions present in the anterior chamber during the first few hours after vitrectomy and grafting, we performed in vitro perfusions of stored human corneas using each irrigant and measured corneal thickness over a 3-hour period. Irrespective of the preservation medium used (McCarey Kaufman, Chondroin Sulfate or Dexsol, all from Chiron Ophthalmics, Irvine, California) or duration of storage (2 or 4 days), corneas irrigated with BSS Plus exhibited significantly (p less than .05) decreased thickness compared with their paired mates irrigated with BSS. In ultrastructural studies performed on postperfusion corneas, there was a tendency toward improved surface morphology in the in vitro BSS Plus-perfused tissue. This study shows that in vitro corneal thickness after preservation is significantly irrigant dependent, with BSS Plus providing the essential ingredients to promote the corneal endothelial pump function.
在角膜移植手术的玻璃体切割部分，可以使用简单的平衡盐溶液（BSS）或含碳酸氢盐缓冲液、谷胱甘肽的商用冲洗液（BSS Plus，爱尔康实验室，美国得克萨斯州沃思堡）。为了模拟玻璃体切割和移植后最初几个小时前房内的情况，我们使用每种冲洗液对保存的人角膜进行体外灌注，并在3小时内测量角膜厚度。无论使用何种保存介质（均来自Chiron Ophthalmics公司，加利福尼亚州欧文市的麦凯里·考夫曼、硫酸软骨素或Dexsol）或保存时间（2天或4天），与用BSS冲洗的配对角膜相比，用BSS Plus冲洗的角膜厚度显著降低（p<0.05）。在对灌注后角膜进行的超微结构研究中，体外经BSS Plus灌注的组织表面形态有改善的趋势。本研究表明，保存后的体外角膜厚度显著依赖于冲洗液，BSS Plus提供了促进角膜内皮泵功能的必需成分。
