Doughty M J, Newlander K, Olejnik O
University of Waterloo, School of Optometry, Ontario, Canada.
J Pharm Pharmacol. 1993 Feb;45(2):102-9. doi: 10.1111/j.2042-7158.1993.tb03692.x.
Corneas from young adult albino rabbits were exposed, in-vitro, to various bicarbonate-free balanced salt solutions under an applied hydrostatic pressure of 20 cmH2O at 35 degrees C for up to 4.5 h. The solutions were buffered with a HEPES-MOPS mixture, phosphate salts or acetate-citrate salts (pH 7.1-7.4). All of these solutions support a net fluid pump activity attributed to the corneal endothelium although the net fluid pump rates were less than those measurable with a bicarbonate-buffered, CO2-equilibrated Ringer solution. Evaluation of the endothelia by scanning electron microscopy revealed no evidence of any acute toxic effect of the balanced salt solutions. Morphometric analyses of cell size and shape revealed a normal mosaic for endothelia exposed to commercial balanced salt solution for 5 h. The results further indicate that exogenous bicarbonate is not required for the net fluid pump function of the mammalian corneal endothelium.
将成年白化兔的角膜在体外暴露于各种无碳酸氢盐的平衡盐溶液中，于35℃、20 cmH₂O的外加静水压力下处理长达4.5小时。这些溶液用HEPES - MOPS混合物、磷酸盐或乙酸盐 - 柠檬酸盐缓冲（pH 7.1 - 7.4）。所有这些溶液都支持归因于角膜内皮的净液体泵活动，尽管净液体泵速率低于用碳酸氢盐缓冲、CO₂平衡的林格氏溶液可测量的速率。通过扫描电子显微镜对内皮进行评估，未发现平衡盐溶液有任何急性毒性作用的证据。对细胞大小和形状的形态计量分析表明，暴露于商用平衡盐溶液5小时的内皮呈现正常镶嵌结构。结果进一步表明，哺乳动物角膜内皮的净液体泵功能不需要外源性碳酸氢盐。
