用于作为一种真菌除草剂的炭疽菌的生产：培养、干燥和储存对回收率和功效的影响。

Production of Colletotrichum truncatum for use as a mycoherbicide: effects of culture, drying and storage on recovery and efficacy.

作者信息

Silman R W, Bothast R J, Schisler D A

机构信息

Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, USDA, Peoria, IL 61604, USA.

出版信息

Biotechnol Adv. 1993;11(3):561-75. doi: 10.1016/0734-9750(93)90025-i.

DOI:10.1016/0734-9750(93)90025-i

PMID:14545677

Abstract

Colletotrichum truncatum (Schwein.) Andrus and Moore NRRL 13737 (= NRRL 18434) is a fungal plant pathogen which shows promise as a bioherbicide against the troublesome weed Sesbania exaltata (Raf.) Rydb. ex A. W. Hill. Previous studies showed similar amounts of spores were produced/ml of medium in liquid and solid-state fermentations. In this study, Colletotrichum truncatum spores were produced in liquid (LC), solid/vermiculite (SV), and solid/perlite-cornmeal-agar (SP). After drying at room temperature with flowing air, SV and SP retained the most viability. Each product was then stored at 4 degrees, 15 degrees, and 25 degrees C. All three products stored at 4 degrees C and SP stored at 15 degrees C retained highest viability. Efficacy based upon assays utilizing equal numbers of viable spores showed SV and SP spores incited more severe disease symptoms than LC spores.

摘要

炭疽菌（Colletotrichum truncatum (Schwein.) Andrus and Moore NRRL 13737 = NRRL 18434）是一种真菌植物病原体，有望成为一种针对麻烦杂草大花田菁（Sesbania exaltata (Raf.) Rydb. ex A. W. Hill）的生物除草剂。先前的研究表明，在液体和固态发酵中，每毫升培养基产生的孢子数量相似。在本研究中，炭疽菌孢子在液体（LC）、固体/蛭石（SV）和固体/珍珠岩-玉米粉-琼脂（SP）中产生。在室温下用流动空气干燥后，SV和SP保留的活力最强。然后将每种产品分别储存在4℃、15℃和25℃。储存在4℃的所有三种产品以及储存在15℃的SP保留的活力最高。基于使用等量活孢子的测定的功效表明，SV和SP孢子引发的病害症状比LC孢子更严重。