Legrand C
J Reprod Fertil. 1977 Nov;51(2):405-8. doi: 10.1530/jrf.0.0510405.
The histochemical distribution of delta5-3beta- and 17beta-hydroxysteroid dehydrogenases was demonstrated in hamster trophoblast between Days 8 and 15 of pregnancy. The delta5-3beta-hydroxysteroid dehydrogenase activity in the ectoplacental trophoblast of 8-day embryos was demonstrated by use of delta5-pregnenolone and dehydroepiandrosterone as substrates; between Days 11 and 15, activity was demonstrated in the trophoblastic giant cells of the placenta and in the intra-arterial trophoblast cells when delta5-pregnenolone was the substrate. Between Days 11 and 15, 17beta-hydroxysteroid activity was present in the spongiotrophoblast, labyrinth, placental giant cells and intra-arterial trophoblast cells, as shown by use of testosterone and oestradiol as substrates. Both enzymes were demonstrated in ectopic trophoblast cells, indicating that these activities are autonomous.
在怀孕第8至15天的仓鼠滋养层中，展示了δ5-3β-和17β-羟基类固醇脱氢酶的组织化学分布。以δ5-孕烯醇酮和脱氢表雄酮为底物，证实了8天胚胎的外胎盘滋养层中存在δ5-3β-羟基类固醇脱氢酶活性；在第11至15天之间，当以δ5-孕烯醇酮为底物时，胎盘的滋养层巨细胞和动脉内滋养层细胞中显示出该活性。以睾酮和雌二醇为底物时显示，在第11至15天之间，海绵滋养层、迷路、胎盘巨细胞和动脉内滋养层细胞中存在17β-羟基类固醇活性。两种酶均在异位滋养层细胞中得到证实，表明这些活性是自主的。
