Valdés Rodolfo, Reyes Biunayki, Alvarez Tatiana, García Janet, Montero José A, Figueroa Alejandro, Gómez Leonardo, Padilla Sigifredo, Geada Déborah, Abrahantes Ma Carmen, Dorta Lamay, Fernández Damián, Mendoza Otto, Ramirez Nadia, Rodriguez Marylin, Pujol Merardo, Borroto Carlos, Brito José
Monoclonal Antibody Department, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
Biochem Biophys Res Commun. 2003 Oct 24;310(3):742-7. doi: 10.1016/j.bbrc.2003.08.149.
This paper provides an evaluation of a plant-derived HBsAg-specific antibody in the immunopurification of the recombinant HBsAg for vaccine purposes. This plant-derived antibody was obtained from different batches of 100-200kg of tobacco leaves and coupled to Sepharose CL-4B with high efficiency. The plant-derived antibody immunoaffinity matrix purification behavior (elution capacity, antigen purity, purification cycles, and ligand leakage) was comparable to that of its mouse-derived monoclonal antibody homolog. This result supports the feasibility of using this plant-derived antibody for the immunopurification of the Hepatitis B surface antigen for human use, opening a new possibility to overcome the constrain of monoclonal antibody production in mice.
本文对一种植物源HBsAg特异性抗体用于重组HBsAg免疫纯化以制备疫苗进行了评估。这种植物源抗体从不同批次100 - 200千克烟草叶中获得，并高效偶联到琼脂糖凝胶CL - 4B上。该植物源抗体免疫亲和基质的纯化行为（洗脱能力、抗原纯度、纯化循环次数和配体泄漏情况）与其小鼠源单克隆抗体类似物相当。这一结果支持了使用这种植物源抗体进行人用乙型肝炎表面抗原免疫纯化的可行性，为克服小鼠单克隆抗体制备的限制开辟了新的可能性。
