从转基因水稻种子中大规模生产功能性人血清白蛋白。
Large-scale production of functional human serum albumin from transgenic rice seeds.
机构信息
Engineering Research Center for Plant Biotechnology and Germplasm Utilization, Ministry of Education, State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China.
出版信息
Proc Natl Acad Sci U S A. 2011 Nov 22;108(47):19078-83. doi: 10.1073/pnas.1109736108. Epub 2011 Oct 31.
Human serum albumin (HSA) is widely used in clinical and cell culture applications. Conventional production of HSA from human blood is limited by the availability of blood donation and the high risk of viral transmission from donors. Here, we report the production of Oryza sativa recombinant HSA (OsrHSA) from transgenic rice seeds. The level of OsrHSA reached 10.58% of the total soluble protein of the rice grain. Large-scale production of OsrHSA generated protein with a purity >99% and a productivity rate of 2.75 g/kg brown rice. Physical and biochemical characterization of OsrHSA revealed it to be equivalent to plasma-derived HSA (pHSA). The efficiency of OsrHSA in promoting cell growth and treating liver cirrhosis in rats was similar to that of pHSA. Furthermore, OsrHSA displays similar in vitro and in vivo immunogenicity as pHSA. Our results suggest that a rice seed bioreactor produces cost-effective recombinant HSA that is safe and can help to satisfy an increasing worldwide demand for human serum albumin.
人血清白蛋白（HSA）在临床和细胞培养应用中被广泛使用。传统上从人血中生产 HSA 受到献血供应的限制和供体病毒传播的高风险的限制。在这里，我们报告了从转基因水稻种子中生产水稻重组 HSA（OsrHSA）。OsrHSA 的水平达到了水稻籽粒总可溶性蛋白的 10.58%。大规模生产 OsrHSA 生成了纯度>99%的蛋白质，生产率为 2.75 g/kg 糙米。OsrHSA 的物理和生化特性表明它与血浆来源的 HSA（pHSA）相当。OsrHSA 促进细胞生长和治疗大鼠肝硬化的效率与 pHSA 相似。此外，OsrHSA 在体外和体内显示出与 pHSA 相似的免疫原性。我们的结果表明，水稻种子生物反应器生产出具有成本效益的重组 HSA，既安全又有助于满足全球对人血清白蛋白日益增长的需求。
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