大肠杆菌O128抗原生物合成簇的序列及一种α-1,2-岩藻糖基转移酶的功能鉴定
Sequence of Escherichia coli O128 antigen biosynthesis cluster and functional identification of an alpha-1,2-fucosyltransferase.
作者信息
Shao Jun, Li Mei, Jia Qiang, Lu Yuquan, Wang Peng George
机构信息
Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
出版信息
FEBS Lett. 2003 Oct 9;553(1-2):99-103. doi: 10.1016/s0014-5793(03)00980-3.
O128 is one of the most common atypical enteropathogenic Escherichia coli isolated from diarrhea patients worldwide. The primary structure of E. coli O128 repeat units has previously been determined as -->3)-beta-D-GalNAc-(1-->4)-alpha-D-Gal-(1-->3)-beta-D-GalNAc-(1-->6)-[alpha-L-Fuc-(1-->2)]-beta-D-Gal-(1--> pentasaccharide. Here we report the complete sequencing of E. coli O128 antigen biosynthesis gene cluster and its flanking regions. Comparative sequence analysis revealed the expected O128 antigen process genes, GDP-fucose biosynthesis genes and four potential glycosyltransferase genes responsible for the assembly of E. coli O128 antigen repeats. WbsJ was shown to encode an alpha-1,2-fucosyltransferase by enzymatic assays and nuclear magnetic resonance spectroscopy analysis.
O128是从全球腹泻患者中分离出的最常见的非典型肠致病性大肠杆菌之一。大肠杆菌O128重复单元的一级结构先前已确定为→3)-β-D-氨基半乳糖-(1→4)-α-D-半乳糖-(1→3)-β-D-氨基半乳糖-(1→6)-[α-L-岩藻糖-(1→2)]-β-D-半乳糖-(1→五糖。在此，我们报告了大肠杆菌O128抗原生物合成基因簇及其侧翼区域的全序列测定。比较序列分析揭示了预期的O128抗原加工基因、GDP-岩藻糖生物合成基因以及负责组装大肠杆菌O128抗原重复序列的四个潜在糖基转移酶基因。通过酶促测定和核磁共振光谱分析表明，WbsJ编码一种α-1,2-岩藻糖基转移酶。
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