大肠杆菌K-12荚膜酸基因簇中岩藻糖合成酶基因的鉴定。
Identification of the fucose synthetase gene in the colanic acid gene cluster of Escherichia coli K-12.
作者信息
Andrianopoulos K, Wang L, Reeves P R
机构信息
Department of Microbiology, The University of Sydney, New South Wales, Australia.
出版信息
J Bacteriol. 1998 Feb;180(4):998-1001. doi: 10.1128/JB.180.4.998-1001.1998.
Abstract
GDP-L-fucose, the substrate for fucosyltransferases for addition of fucose to polysaccharides or glycoproteins in both procaryotes and eucaryotes, is made from GDP-D-mannose. L-Fucose is a component of bacterial surface antigens, including the extracellular polysaccharide colanic acid produced by most Escherichia coli strains. We previously sequenced the E. coli colanic acid gene cluster and identified one of the GDP-L-fucose biosynthetic pathway genes, gmd. We report here the identification of the gene (fcl), located downstream of gmd, encoding the fucose synthetase.
摘要
GDP-L-岩藻糖是原核生物和真核生物中用于将岩藻糖添加到多糖或糖蛋白上的岩藻糖基转移酶的底物，它由GDP-D-甘露糖合成。L-岩藻糖是细菌表面抗原的一个组成部分，包括大多数大肠杆菌菌株产生的细胞外多糖结肠酸。我们之前对大肠杆菌结肠酸基因簇进行了测序，并鉴定出GDP-L-岩藻糖生物合成途径基因之一gmd。我们在此报告位于gmd下游、编码岩藻糖合成酶的基因（fcl）的鉴定结果。
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