Bureau-Chalot F, Piednoir E, Pierrat C, Santerne B, Bajolet O
Laboratoire de bactériologie-virologie-hygiène, CHU de Reims, avenue du Général-Koenig, 51092 Reims cedex, France.
Arch Pediatr. 2003 Oct;10(10):882-6. doi: 10.1016/s0929-693x(03)00452-4.
We report an outbreak of Burkholderia cepacia respiratory tract infection and colonization in an intensive pediatric care unit.P PATIENTS AND METHODS: Between February and December 1999, B. cepacia was isolated from five children hospitalized in this unit. We reviewed the charts of the patients, evaluated the antiseptics use and the disinfection practices for reusable patient care equipment. An environmental study was conducted and comparison of B. cepacia was performed with genotypic method (RAPD).
All patients were mechanically ventilated and had received large spectrum antibiotics. The disinfection procedure for reusable equipment was not respected and some single-dose of antiseptics solutions were used for several patients. B. cepacia was not found in 34 environmental samples. The RAPD assay revealed that all five isolates had identical DNA profiles.
Despite the investigation the source of the B. cepacia clone in this nosocomial outbreak remained unknown, but antiseptics use and disinfection practices were revised. No new B. cepacia infections were identified after control measures were implemented.
我们报告了一家儿科重症监护病房爆发洋葱伯克霍尔德菌呼吸道感染及定植事件。
1999年2月至12月期间，该病房收治的5名儿童分离出洋葱伯克霍尔德菌。我们查阅了患者病历，评估了防腐剂的使用情况以及可重复使用的患者护理设备的消毒措施。进行了环境研究，并采用基因分型方法（随机扩增多态性DNA分析）对分离出的洋葱伯克霍尔德菌进行比较。
所有患者均接受机械通气，且均使用了广谱抗生素。可重复使用设备的消毒程序未得到遵守，一些单剂量的防腐剂溶液被多名患者使用。34份环境样本中未发现洋葱伯克霍尔德菌。随机扩增多态性DNA分析显示，所有5株分离菌具有相同的DNA图谱。
尽管进行了调查，但此次医院感染爆发中洋葱伯克霍尔德菌克隆的来源仍不清楚，但已对防腐剂的使用和消毒措施进行了修订。实施控制措施后，未发现新的洋葱伯克霍尔德菌感染病例。
