Gupta Sanjay, Sodhani Pushpa, Chachra Krishan Lal
Division of Cytopathology, Institute of Cytology and Preventive Oncology, New Delhi, India.
Obstet Gynecol. 2003 Oct;102(4):761-4. doi: 10.1016/s0029-7844(03)00771-3.
To evaluate the effect of rehydration of air-dried cervical smears on staining quality and to find out whether rehydration techniques can be adopted as an alternative method, especially in high-volume, resource-limited settings.
A total of 950 paired wet-fixed and air-dried-rehydrated cervical smears collected by paramedical workers from an urban slum of Delhi, India, were compared for staining quality by assessing different cytoplasmic and nuclear parameters.
The staining quality in air-dried-rehydrated smears was satisfactory and comparable to wet-fixed smears (61.3% versus 63.6%). The staining was superior in rehydrated smears in 26.5%, as compared with 15.4% in wet-fixed ones (P <.001). Unsatisfactory staining was observed in 12.2% of rehydrated smears, as compared with 21% of wet-fixed smears. The smear background was cleaner in rehydrated smears. Red blood cells were observed in only 3% of rehydrated smears, in comparison to 12% in wet-fixed ones (P <.001).
Rehydration of air-dried cervical smears is a suitable alternative to wet-fixed smears. The staining quality is either the same as or better than wet-fixed smears, and the unsatisfactory rate is lower. This technique is simple and can be conveniently adopted in resource-limited settings.
评估风干宫颈涂片复水对染色质量的影响，并探究复水技术是否可作为一种替代方法，尤其是在高工作量、资源有限的环境中。
对印度德里一个城市贫民窟的辅助医务人员收集的950对湿固定和风干复水宫颈涂片，通过评估不同的细胞质和细胞核参数来比较染色质量。
风干复水涂片的染色质量令人满意，与湿固定涂片相当（分别为61.3%和63.6%）。复水涂片染色效果优于湿固定涂片的比例为26.5%，而湿固定涂片为15.4%（P<.001）。复水涂片中有12.2%染色不满意，而湿固定涂片为21%。复水涂片的涂片背景更干净。复水涂片中仅3%观察到红细胞，而湿固定涂片为12%（P<.001）。
风干宫颈涂片复水是湿固定涂片的合适替代方法。染色质量与湿固定涂片相同或更好，不满意率更低。该技术简单，可在资源有限的环境中方便地采用。
