通过高效液相色谱法定量测定尿液中来自胶原蛋白的羟基吡啶交联物。

Quantitation of urinary hydroxypyridinium cross-links from collagen by high-performance liquid chromatography.

作者信息

Kollerup G, Thamsborg G, Bhatia H, Sørensen O H

机构信息

Department of Medicine, Sundby Hospital, Copenhagen, Denmark.

出版信息

Scand J Clin Lab Invest. 1992 Nov;52(7):657-62. doi: 10.3109/00365519209115510.

DOI:10.3109/00365519209115510

PMID:1455159

Abstract

Pyridinoline and deoxypyridinoline are intermolecular cross-links in mature collagen in bone and cartilage. The urinary excretion of the two compounds correlates well to bone turnover. A fast, sensitive, and accurate isocratic ion-pairing reverse-phase high-performance liquid chromatography method for measurement of pyridinoline and deoxypyridinoline in urine has been established. Intra- and inter-assay precision were 5-7% and 12-14%, respectively. Recovery for pyridinoline was 97.4% and for deoxypyridinoline 94.3%. The detection limit was 0.4 pmol. Pyridinoline:creatinine and deoxypyridinoline: creatinine ratios in healthy subjects, were 38.8 nmol:mmol and 13.0 nmol:mmol, respectively. Increased values of both cross-links were observed in children, in the age group 20-29 in both sexes, and in post-menopausal women.

摘要

吡啶啉和脱氧吡啶啉是骨骼和软骨中成熟胶原蛋白的分子间交联物。这两种化合物的尿排泄量与骨转换密切相关。已建立一种快速、灵敏且准确的等度离子对反相高效液相色谱法，用于测定尿液中的吡啶啉和脱氧吡啶啉。批内和批间精密度分别为5%-7%和12%-14%。吡啶啉的回收率为97.4%，脱氧吡啶啉的回收率为94.3%。检测限为0.4皮摩尔。健康受试者中吡啶啉与肌酐、脱氧吡啶啉与肌酐的比值分别为38.8纳摩尔:毫摩尔和13.0纳摩尔:毫摩尔。在儿童、20-29岁年龄段的男女以及绝经后女性中，均观察到这两种交联物的值升高。