Watanabe Keiro, Okada Kiyotaka
Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
Plant Cell. 2003 Nov;15(11):2592-602. doi: 10.1105/tpc.015214. Epub 2003 Oct 10.
Our previous studies showed that a member of the YABBY gene family, FILAMENTOUS FLOWER (FIL), plays a role in specifying the abaxial side tissues in the development of lateral organs such as cotyledons, leaves, young flower buds, and flower organs. We examined the expression pattern of FIL and found a temporal change of expression domains in the developmental process of the floral meristem. We also examined the cis control regions by constructing a series of transgenic plants that carry green fluorescent protein under the control of the FIL promoter with several types of deletions, base changes, and tandem repeats and showed that the unique expression pattern is dependent on at least two cis-acting elements in the 5' regulatory region. One element proximal to the FIL gene would be responsible for the expression of both the abaxial and adaxial sides, and the other element of the 12-bp sequence would work to repress expression on the adaxial side.
我们之前的研究表明，YABBY基因家族的一个成员，丝状花（FIL），在子叶、叶片、幼花芽和花器官等侧生器官发育过程中确定远轴侧组织方面发挥作用。我们检测了FIL的表达模式，发现在花分生组织发育过程中表达域存在时间变化。我们还通过构建一系列转基因植物来检测顺式调控区域，这些转基因植物在FIL启动子的控制下携带绿色荧光蛋白，具有几种类型的缺失、碱基变化和串联重复，结果表明独特的表达模式至少依赖于5'调控区域中的两个顺式作用元件。FIL基因附近的一个元件负责远轴侧和近轴侧的表达，而12bp序列的另一个元件则起到抑制近轴侧表达的作用。
