传染性胃肠炎冠状病毒与刷状缘膜唾液糖蛋白的结合。
Binding of transmissible gastroenteritis coronavirus to brush border membrane sialoglycoproteins.
作者信息
Schwegmann-Wessels Christel, Zimmer Gert, Schröder Bernd, Breves Gerhard, Herrler Georg
机构信息
Institut für Virologie, Tierärztliche Hochschule Hannover, 30559 Hannover, Germany.
出版信息
J Virol. 2003 Nov;77(21):11846-8. doi: 10.1128/jvi.77.21.11846-11848.2003.
Abstract
Transmissible gastroenteritis coronavirus (TGEV) is a porcine pathogen causing enteric infections that are lethal for suckling piglets. The enterotropism of TGEV is connected with the sialic acid binding activity of the viral surface protein S. Here we show that, among porcine intestinal brush border membrane proteins, TGEV recognizes a mucin-type glycoprotein designated MGP in a sialic acid-dependent fashion. Virus binding assays with cryosections of the small intestine from a suckling piglet revealed the binding of TGEV to mucin-producing goblet cells. A nonenteropathogenic mutant virus that lacked a sialic acid binding activity was unable to bind to MGP and to attach to goblet cells. Our results suggest a role of MGP in the enteropathogenicity of TGEV.
摘要
传染性胃肠炎冠状病毒（TGEV）是一种猪病原体，可引起肠道感染，对哺乳仔猪具有致死性。TGEV的肠嗜性与病毒表面蛋白S的唾液酸结合活性有关。在此我们表明，在猪肠道刷状缘膜蛋白中，TGEV以唾液酸依赖的方式识别一种名为MGP的粘蛋白型糖蛋白。用哺乳仔猪小肠冰冻切片进行的病毒结合试验显示TGEV与产生粘蛋白的杯状细胞结合。一种缺乏唾液酸结合活性的非肠道致病性突变病毒无法与MGP结合，也无法附着于杯状细胞。我们的结果表明MGP在TGEV的肠道致病性中发挥作用。
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本文引用的文献
1
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J Virol. 2002 Jun;76(12):6037-43. doi: 10.1128/jvi.76.12.6037-6043.2002.
2
N-glycans of F protein differentially affect fusion activity of human respiratory syncytial virus.
J Virol. 2001 May;75(10):4744-51. doi: 10.1128/JVI.75.10.4744-4751.2001.
3
Sialic acid binding activity of transmissible gastroenteritis coronavirus affects sedimentation behavior of virions and solubilized glycoproteins.
J Virol. 2001 Jan;75(2):844-9. doi: 10.1128/JVI.75.2.844-849.2001.
4
Characterization of the sialic acid binding activity of transmissible gastroenteritis coronavirus by analysis of haemagglutination-deficient mutants.
J Gen Virol. 2000 Feb;81(Pt 2):489-96. doi: 10.1099/0022-1317-81-2-489.
5
Phosphate transport in pig proximal small intestines during postnatal development: lack of modulation by calcitriol.
Endocrinology. 1998 Apr;139(4):1500-7. doi: 10.1210/endo.139.4.5922.
6
Point mutations in the S protein connect the sialic acid binding activity with the enteropathogenicity of transmissible gastroenteritis coronavirus.
J Virol. 1997 Apr;71(4):3285-7. doi: 10.1128/JVI.71.4.3285-3287.1997.
7
Transmissible gastroenteritis coronavirus, but not the related porcine respiratory coronavirus, has a sialic acid (N-glycolylneuraminic acid) binding activity.
J Virol. 1996 Aug;70(8):5634-7. doi: 10.1128/JVI.70.8.5634-5637.1996.
8
Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose.
J Biochem Biophys Methods. 1984 Dec;10(3-4):203-9. doi: 10.1016/0165-022x(84)90040-x.
9
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
10
Residues involved in the antigenic sites of transmissible gastroenteritis coronavirus S glycoprotein.
Virology. 1991 Jul;183(1):225-38. doi: 10.1016/0042-6822(91)90135-x.
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