Capelari Marina, Fungaro Maria Helena P
Instituto de Botânica, Caixa Postal 4005, CEP 01061-970, São Paulo, SP, Brasil.
Mycol Res. 2003 Sep;107(Pt 9):1050-4. doi: 10.1017/s0953756203008153.
Isolates of Pleurotus cystidiosus and P. smithii were studied to verifie the occurrence of P. cystidiosus instead of P. smithii in South America. The two species are mainly separated by the growth rate of the anamorph in culture, the morphology of the anamorph and teleomorph, intercompatibility tests, and genetic variability. In order to see if the isolate found in Brazil and previously identified as Antromycopsis macrocarpa (the anamorph of P. cystidiosus) belongs to P. cystidiosus, a species with a world wide distribution, or to P. smithii which is restricted to Mexico and South America, or if P. cyistidiosus and P. smithii are the same species, isolates of different geographic origins were studied. Growth rate in culture, mono-dikaryotic matings, and genetic variability determined by RADP were investigated. The results show that the criteria used to separate the two species are unsatisfactory, and that P. smithii should be considered a synonym of P. cystidiosus; this extends the distribution of this later species to Central and South America.
对囊状侧耳和平展侧耳的分离菌株进行了研究，以验证在南美洲出现的是囊状侧耳而非平展侧耳。这两个物种主要通过培养物中无性型的生长速率、无性型和有性型的形态、互交亲和性测试以及遗传变异性来区分。为了确定在巴西发现的、先前被鉴定为大孢拟暗孔菌（囊状侧耳的无性型）的分离菌株是属于囊状侧耳（一种分布于世界各地的物种），还是属于仅局限于墨西哥和南美洲的平展侧耳，或者囊状侧耳和平展侧耳是否为同一物种，对不同地理来源的分离菌株进行了研究。研究了培养物中的生长速率、单核 -双核交配以及由随机扩增多态性DNA（RAPD）确定的遗传变异性。结果表明，用于区分这两个物种的标准并不令人满意，平展侧耳应被视为囊状侧耳的同义词；这将后一个物种的分布范围扩展到了中美洲和南美洲。
