Szeto A C, Pérez-Rosado J, Ferrer-Rodríguez I, Vega J, Torruella-Thillet C, Serrano A E
Dept. of Microbiology/Medical Zoology, School of Medicine, University of Puerto Rico, P.O. Box 365067, San Juan, Puerto Rico, 00936-5067.
Parasitol Res. 2004 Jan;92(1):1-11. doi: 10.1007/s00436-003-1001-8. Epub 2003 Oct 17.
The ATP-binding cassette (ABC) proteins are one of the largest evolutionarily conserved families. They are characterized by the presence of highly conserved nucleotide-binding sites (NBS). In the present study, we identified ABC genes in rodent Plasmodia. We queried the Plasmodium yoelii genome with the ABC signature motif and retrieved 15 contigs. Sequences were classified into seven ABC families by BLAST comparison. Conservation of the five signature ABC motifs in the P. yoelii contigs was examined by multi-alignment of the NBS. Expression of the ABC genes was examined during the blood stages of P. yoelii and P. berghei and the hepatocytic stages of P. yoelii. Our results with RT-PCR on total RNA from blood stages demonstrated the expression of 14 ABC genes in P. yoelii and ten in P. berghei. In P. yoelii hepatocytic stages, the expression of four ABC genes was detected.
ATP结合盒（ABC）蛋白是进化上保守的最大蛋白家族之一。它们的特征是存在高度保守的核苷酸结合位点（NBS）。在本研究中，我们在啮齿类疟原虫中鉴定出ABC基因。我们用ABC特征基序查询约氏疟原虫基因组，检索到15个重叠群。通过BLAST比较将序列分为七个ABC家族。通过NBS的多序列比对检查约氏疟原虫重叠群中五个特征性ABC基序的保守性。在约氏疟原虫和伯氏疟原虫的血液阶段以及约氏疟原虫的肝细胞阶段检测ABC基因的表达。我们对血液阶段总RNA进行RT-PCR的结果表明，约氏疟原虫中有14个ABC基因表达，伯氏疟原虫中有10个ABC基因表达。在约氏疟原虫肝细胞阶段，检测到四个ABC基因的表达。
