Xia Zhunan, Cianflone Katherine
Mike Rosenbloom Laboratory for Cardiovascular Research, McGill University Health Centre, Montreal, Quebec, Canada.
Metabolism. 2003 Oct;52(10):1360-6. doi: 10.1016/s0026-0495(03)00254-3.
Recent reports have suggested a link between acylation-stimulating protein (ASP) and complement C3 with obesity, insulin resistance, coronary artery disease, and hyperlipidemia. Our aim was to examine the mRNA expression of C3 and other factors related to ASP production (such as factor B and adipsin) in adipose tissue. The influence of gender and obesity was examined in subcutaneous (SC) and omental (OM) tissues from 16 males and 16 females with body mass index (BMI) from 20 to 54 kg/m(2). The results demonstrate that factor B mRNA expression is higher in males than females in both SC and OM tissues. In female SC tissue, C3 and adipsin mRNA decrease with increasing BMI (r = 0.557, P =.025 and r = 0.717 P =.002, respectively), with no change in factor B. By contrast, in males there was a pronounced increase in C3, adipsin, and factor B in OM tissue with increasing BMI (r = 0.759 P =.001, r = 0.650 P =.006, and r = 0.568 P =.022, respectively). Of note, however, in both men and women there was a marked increase in the OM/SC ratio of C3 and adipsin with increasing BMI. These results suggest that in female SC adipose tissue, there is downregulation of factors related to ASP production in obesity, perhaps to limit further expansion of adipose tissue. In males, there is increased expression in OM tissue. In addition, relative OM/SC expression increases with obesity and these changes may contribute to the development of visceral adipose tissue.
近期报告表明，酰化刺激蛋白（ASP）和补体C3与肥胖、胰岛素抵抗、冠状动脉疾病及高脂血症之间存在关联。我们的目的是检测脂肪组织中C3及其他与ASP产生相关因子（如B因子和脂肪酶）的mRNA表达。对16名男性和16名女性（体重指数[BMI]为20至54kg/m²）的皮下（SC）和网膜（OM）组织进行研究，以考察性别和肥胖的影响。结果显示，在SC和OM组织中，男性的B因子mRNA表达均高于女性。在女性SC组织中，C3和脂肪酶mRNA随BMI增加而降低（r分别为0.557，P = 0.025和r = 0.717，P = 0.002），而B因子无变化。相比之下，在男性中，OM组织中C3、脂肪酶和B因子随BMI增加而显著升高（r分别为0.759，P = 0.001；r = 0.650，P = 0.006；r = 0.568，P = 0.022）。然而，值得注意的是，无论男性还是女性，C3和脂肪酶的OM/SC比值均随BMI增加而显著升高。这些结果表明，在女性SC脂肪组织中，肥胖时与ASP产生相关的因子会下调，这可能是为了限制脂肪组织的进一步扩张。在男性中，OM组织中的表达增加。此外，相对OM/SC表达随肥胖增加，这些变化可能有助于内脏脂肪组织的发展。
