Vogel W C, Bierman E L
Medical Service, Veterans Administration Hospital and Department of Medicine, University of Washington School of Medicine, Seattle, USA.
J Lipid Res. 1967 Jan;8(1):46-53.
Lecithinase activity in post-heparin serum has been demonstrated. Phosphatidyl choline (PC) can be degraded to lysophosphatidyl choline and fatty acids at a rate of more than 1 micromole/hr per ml of serum in an incubation system containing PC, 0.1 M glycine-NaOH buffer (pH 9.6), and deoxycholate. This activity cannot be found in serum obtained prior to the injection of heparin. Post-heparin serum lecithinase can be distinguished from the heat-stable pancreatic lecithinase by the markedly different effects of heat, paraoxon, and EDTA, and from serum lecithin:cholesterol acyltransferase by the differential effect of p-hydroxymercuribenzoate. In contrast to the acyltransferase and to pancreatic lecithinase, which are active at the beta (C-2) position of lecithin, post-heparin serum lecithinase is active at alpha' (C-1) position.
已证实肝素化后血清中存在卵磷脂酶活性。在含有磷脂酰胆碱（PC）、0.1M甘氨酸 - 氢氧化钠缓冲液（pH 9.6）和脱氧胆酸盐的孵育体系中，磷脂酰胆碱可降解为溶血磷脂酰胆碱和脂肪酸，降解速率为每毫升血清每小时超过1微摩尔。在注射肝素之前获得的血清中未发现这种活性。肝素化后血清卵磷脂酶可通过热、对氧磷和乙二胺四乙酸（EDTA）的显著不同作用与热稳定的胰腺卵磷脂酶区分开来，也可通过对羟基汞苯甲酸的差异作用与血清卵磷脂：胆固醇酰基转移酶区分开来。与在卵磷脂β（C - 2）位具有活性的酰基转移酶和胰腺卵磷脂酶不同，肝素化后血清卵磷脂酶在α'（C - 1）位具有活性。
