Roach G D, Burgess H, Lamond N, Dorrian J, Holmes A, Fletcher A, McCulloch K, Dawson D
Centre for Sleep Research, University of South Australia, Adelaide, Australia.
J Hum Ergol (Tokyo). 2001 Dec;30(1-2):255-60.
In most studies, the magnitude and rate of adaptation to various night work schedules is assessed using core body temperature as the marker of circadian phase. The aim of the current study was to assess adaptation to a simulated night work schedule using salivary dim light melatonin onset (DLMO) as an alternative circadian phase marker. It was hypothesised that the night work schedule would result in a phase delay, manifest in relatively later DLMO, but that this delay would be somewhat inhibited by exposure to natural light. Participants worked seven consecutive simulated 8-hour night shifts (23:00-07:00 h). By night 7, there was a mean cumulative phase delay of 5.5 hours, equivalent to an average delay of 0.8 hours per day. This indicates that partial circadian adaptation occurred in response to the simulated night work schedule. The radioimmunoassay used in the current study provides a sensitive assessment of melatonin concentration in saliva that can be used to determine DLMO, and thus provides an alternative phase marker to core body temperature, at least in laboratory studies.
在大多数研究中，使用核心体温作为昼夜节律相位的标志物来评估对各种夜班工作时间表的适应程度和速率。本研究的目的是使用唾液褪黑素暗光起始（DLMO）作为替代的昼夜节律相位标志物来评估对模拟夜班工作时间表的适应情况。研究假设夜班工作时间表会导致相位延迟，表现为DLMO相对较晚出现，但这种延迟会因暴露于自然光而受到一定程度的抑制。参与者连续进行了七个模拟的8小时夜班（23:00 - 07:00）。到第7个晚上，平均累积相位延迟为5.5小时，相当于每天平均延迟0.8小时。这表明对模拟夜班工作时间表产生了部分昼夜节律适应。本研究中使用的放射免疫测定法能对唾液中的褪黑素浓度进行灵敏评估，可用于确定DLMO，因此至少在实验室研究中提供了一种替代核心体温的相位标志物。
