Wu Zhong, Dian Ke, Shi Yingkang, Wan Changxiu, Zhao Qiang
Department of Thoracic and Cardiovascular Surgery, West China Hospital of Sichuan University, Chengdu 610041, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2005 Jun;22(3):456-8.
In this study for exploring the effect of RGD peptide on adhesive stability of endothelial cells biomaterial surface, all materials were divided into three groups, RGD group (PET covalently grafted synthetic RGD peptides), control group (PET precoated with fibronectin) and blank group (Non-coated surface). Cultured human umbilical vein endothelial cells (HUVECs) were seeded on the materials, then adhesive stability of HUVECs on the varied PET surfaces was observed under steady flow condition, and effects of shear stress and shear time on adherent cells were compared. The results showed that the resistance adherent endothelial cells to detachment by flow was shear stress and shear time dependent. Comparison three groups under the same condition revealed that the ECs retention rates of RGD-grafted or fibronectin-coated group were much higher than that of the non-coated group. Under 8.19 dyne/cm2 shear stress after 4h, retention rates were 13.73% (blank group), 43.33% (RGD group) and 40.75% (control group) respectively. These data indicated that RGD peptide can improve the adhesive stability of endothelial cell on biomaterial and the effect of RGD in vivo needs further studies.
在本研究中，为了探究RGD肽对内皮细胞与生物材料表面黏附稳定性的影响，所有材料被分为三组：RGD组（聚对苯二甲酸乙二酯共价接枝合成RGD肽）、对照组（聚对苯二甲酸乙二酯预包被纤连蛋白）和空白组（未包被表面）。将培养的人脐静脉内皮细胞（HUVECs）接种于这些材料上，然后在稳定流动条件下观察HUVECs在不同聚对苯二甲酸乙二酯表面的黏附稳定性，并比较剪切应力和剪切时间对贴壁细胞的影响。结果表明，流动状态下内皮细胞抵抗脱离的能力取决于剪切应力和剪切时间。在相同条件下对三组进行比较发现，接枝RGD或包被纤连蛋白组的内皮细胞保留率远高于未包被组。在8.19达因/平方厘米剪切应力作用4小时后，保留率分别为13.73%（空白组）、43.33%（RGD组）和40.75%（对照组）。这些数据表明，RGD肽可提高内皮细胞在生物材料上的黏附稳定性，而RGD在体内的作用有待进一步研究。
