Callan M B, Giger U, Oakley D A, Scotti M V, Shofer F S
Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104-6010.
Am J Vet Res. 1992 Oct;53(10):1760-4.
In veterinary medicine, PCV determined by centrifugation of blood in a microhematocrit tube is the most common clinical test used to initially assess and monitor anemic and polycythemic animals. In contrast, blood hemoglobin (Hb) concentration, rather than PCV, is generally determined in human patients. One automated system photometrically measures blood Hb concentration after conversion of Hb to azide methemoglobin without dilution and was found to be a simple and accurate instrument for use in human medicine. We evaluated the system for its accuracy in measuring blood Hb concentration in animals by comparing it with standard techniques and for its suitability in veterinary practice. Blood samples, anticoagulated with potassium EDTA, from 78 healthy animals (33 dogs, 17 cats, 13 horses, and 15 cows) and 58 dogs and 4 cats with various blood abnormalities (10 anemia, 11 polycythemia, 21 lipemia, 16 leukocytosis, and 6 icterus) were analyzed. In all species, blood Hb concentration of healthy animals determined by the system was comparable to that measured by standard cyanmethemoglobin methods (ie, an automated counter; rI = 0.987 to 0.998 and a hemoglobin kit, rI = 0.946 to 0.993). The aforementioned system also yielded similar values to those obtained by use of standard methods in anemic, polycythemic, and icteric dogs and cats. Moreover, the system reads the absorbance at 2 wavelengths to correct for turbidity, and therefore, accurately measured Hb concentration in blood samples with severe lipemia (triglycerides concentration > 500 mg/dl) and marked leukocytosis (> 50,000 WBC/microliter), whereas other standard Hb techniques are known to give falsely high results.(ABSTRACT TRUNCATED AT 250 WORDS)
在兽医学中，通过在微量血细胞比容管中离心血液来测定的红细胞压积（PCV）是用于初步评估和监测贫血及红细胞增多动物的最常见临床检测方法。相比之下，人类患者通常测定的是血液血红蛋白（Hb）浓度，而非PCV。一种自动化系统在不稀释的情况下将Hb转化为叠氮高铁血红蛋白后，通过光度法测量血液Hb浓度，被发现是一种用于人类医学的简单且准确的仪器。我们通过将该系统与标准技术进行比较，评估其在测量动物血液Hb浓度方面的准确性以及在兽医实践中的适用性。分析了来自78只健康动物（33只狗、17只猫、13匹马和15头牛）以及58只患有各种血液异常（10例贫血、11例红细胞增多症、21例脂血症、16例白细胞增多症和6例黄疸）的狗和4只猫的用乙二胺四乙酸钾抗凝的血液样本。在所有物种中，该系统测定的健康动物血液Hb浓度与通过标准氰化高铁血红蛋白方法（即自动血细胞计数器；相关系数rI = 0.987至0.998，以及血红蛋白试剂盒，rI = 0.946至0.993）测量的结果相当。上述系统在贫血、红细胞增多症和黄疸的狗和猫中得出的值也与使用标准方法获得的值相似。此外，该系统读取两个波长处的吸光度以校正浊度，因此能够准确测量严重脂血症（甘油三酯浓度> 500 mg/dl）和显著白细胞增多症（> 50,000个白细胞/微升）的血液样本中的Hb浓度，而其他标准Hb技术已知会给出错误的高结果。（摘要截短于250字）
