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2'-脱氧-2',2''-二氟-β-D-呋喃核糖基和2'-脱氧-2',2''-二氟-α-D-呋喃核糖基寡核苷酸的合成及物理化学性质

Synthesis and physicochemical properties of 2'-deoxy-2',2''-difluoro-beta-D-ribofuranosyl and 2'-deoxy-2',2''-difluoro-alpha-D-ribofuranosyl oligonucleotides.

作者信息

Viazovkina Ekaterina, Mangos Maria M, Damha Masad J

机构信息

Department of Chemistry, McGill University, Montreal, QC, Canada.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1251-4. doi: 10.1081/NCN-120022848.

DOI:10.1081/NCN-120022848
PMID:14565392
Abstract

We present procedures for nucleoside and oligonucleotide synthesis, binding affinity (Tm) and structural analysis (CD spectra) of 2'-deoxy-2',2''-difluoro-alpha-D-ribofuranosyl and 2'-deoxy-2',2''-difluoro-beta-D-ribofuranosyl oligothymidylates. Possible reasons for the thermal instability of duplexes formed between these compounds and RNA or DNA targets are discussed.

摘要

我们展示了2'-脱氧-2',2''-二氟-α-D-呋喃核糖基和2'-脱氧-2',2''-二氟-β-D-呋喃核糖基寡聚胸苷酸的核苷和寡核苷酸合成、结合亲和力（熔解温度）及结构分析（圆二色光谱）的方法。讨论了这些化合物与RNA或DNA靶标形成的双链体热不稳定性的可能原因。

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