用于DNA阵列核酸检测的通用标记化学方法。
Universal labeling chemistry for nucleic acid detection on DNA-arrays.
作者信息
Bernal-Méndez E, Tora C, Sothier I, Kotera M, Troesch A, Laayoun A
机构信息
BioMérieux, Advanced Technology, Marcy l'Etoile, France.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1647-9. doi: 10.1081/NCN-120023090.
PMID:14565486
Abstract
We show here a new and efficient aqueous chemistry for labeling of any class of nucleic acids for their detection on DNA chip. The labels contain a diazo function as reactive moiety and biotin as detectable unit. The highly selective reaction of diazo group on the phosphate does not disrupt base pairing recognition and hybridization specificity.
摘要
我们在此展示了一种全新且高效的水相化学方法，用于标记任何一类核酸，以便在DNA芯片上进行检测。这些标记物含有一个作为反应性部分的重氮官能团和一个作为可检测单元的生物素。重氮基团与磷酸根的高选择性反应不会破坏碱基配对识别和杂交特异性。
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