2',5'-连接的四核糖核苷酸环的结构：一种新型RNA基序。

Structure of 2',5'-linked tetraribonucleotide loops: a novel RNA motif.

作者信息

Hannoush Rami N, Denisov Alexei Yu, Gehring Kalle, Damha Masad J

机构信息

Department of Chemistry, McGill University, Montreal, Quebec, Canada.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1687-90. doi: 10.1081/NCN-120023114.

DOI:10.1081/NCN-120023114

PMID:14565496

Abstract

We report on the three dimensional structure of an RNA hairpin containing a 2',5'-linked tetraribonucleotide loop, namely, 5'-rGGAC(UUCG)GUCC-3' (where UUCG = U(2'p5')U(2'p5')C(2'p5')G(2'p5')). We show that the 2',5'-linked RNA loop adopts a conformation that is quite different from that previously observed for the native 3',5'-linked RNA loop. The 2',5'-RNA loop is stabilized by (a) U:G wobble base pairing, with both bases in the anti conformation, (b) extensive base stacking, and (c) sugar-base contacts, all of which contribute to the extra stability of this hairpin structure.

摘要

我们报道了一种含有2',5'-连接的四核糖核苷酸环的RNA发夹结构，即5'-rGGAC(UUCG)GUCC-3'（其中UUCG = U(2'p5')U(2'p5')C(2'p5')G(2'p5')）。我们发现，2',5'-连接的RNA环所采用的构象与之前观察到的天然3',5'-连接的RNA环的构象有很大不同。2',5'-RNA环通过以下方式得以稳定：(a) U:G摆动碱基对，两个碱基均处于反式构象；(b) 广泛的碱基堆积；以及(c) 糖-碱基接触，所有这些都有助于该发夹结构的额外稳定性。