胶束电动毛细管色谱法测定女贞子中齐墩果酸和熊果酸异构体

MECC determination of oleanolic acid and ursolic acid isomers in Ligustrum lucidum Ait.

作者信息

Liu Haixing, Shi Yuhua, Wang Dexian, Yang Gengliang, Yu Aimin, Zhang Hanqi

机构信息

College of Chemistry, Jilin University, Changchun 130023, PR China.

出版信息

J Pharm Biomed Anal. 2003 Jul 14;32(3):479-85. doi: 10.1016/s0731-7085(03)00235-8.

DOI:10.1016/s0731-7085(03)00235-8

PMID:14565552

Abstract

In this paper, a novel method to separate and determine oleanolic acid and ursolic acid isomers in Ligustrum lucidum Ait was studied by micellar electrokinetic capillary chromatography (MECC). The baseline separation of the two analytes were obtained on the condition that the buffer contained 15 mmol/l disodium hydrogen phosphate, 15 mmol/l disodium tetreborate, 10 mmol/l SDS and 5% (v/v) alcohol. The contents of the oleanolic acid and ursolic acid were determined in L. lucidum Ait, Folium photiniae and Flos campsis; they were 78.3 (R.S.D. = 2.75%) and 20.7 mg/g (R.S.D. = 2.97%), 27.9 (R.S.D. = 3.67%) and 79.8 mg/g (R.S.D. = 3.44%), 65.5 (R.S.D. = 3.73%) and 60.4 mg/g (R.S.D. = 4.06%) (n = 5), respectively. The recoveries of the analytes in the extract of L. lucidum Ait were 102%, (R.S.D. = 2.85%) for oleanolic acid and 104% (R.S.D. = 3.21%) for ursolic acid (n = 5). With the emphasis on the effects of SDS and alcohol concentrations on the separation of the isomers were investigated.

摘要

本文采用胶束电动毛细管色谱法（MECC）研究了一种分离和测定女贞子中齐墩果酸和熊果酸异构体的新方法。在缓冲液含有15 mmol/l磷酸氢二钠、15 mmol/l四硼酸钠、10 mmol/l十二烷基硫酸钠和5%（v/v）乙醇的条件下，实现了两种分析物的基线分离。测定了女贞子、石楠叶和凌霄花中齐墩果酸和熊果酸的含量；它们分别为78.3（相对标准偏差=2.75%）和20.7 mg/g（相对标准偏差=2.97%）、27.9（相对标准偏差=3.67%）和79.8 mg/g（相对标准偏差=3.44%）、65.5（相对标准偏差=3.73%）和60.4 mg/g（相对标准偏差=4.06%）（n = 5）。女贞子提取物中分析物的回收率，齐墩果酸为102%（相对标准偏差=2.85%），熊果酸为104%（相对标准偏差=3.21%）（n = 5）。重点研究了十二烷基硫酸钠和乙醇浓度对异构体分离的影响。