通过连续培养对盘基网柄菌固定状态下的行为进行分析。

Analysis of the behaviour of Dictyostelium discoideum in immobilised state by means of continuous cultivation.

作者信息

Beshay U, Friehs K, Azzam A-E-M, Flaschel E

机构信息

Faculty of Technology, Bielefeld University, 33594 Bielefeld, Germany.

出版信息

Bioprocess Biosyst Eng. 2003 Dec;26(2):117-22. doi: 10.1007/s00449-003-0339-8. Epub 2003 Oct 18.

DOI:10.1007/s00449-003-0339-8

PMID:14566554

Abstract

The social amoeba Dictyostelium discoideum is amenable to cultivation in the immobilised state most simply by colonisation of porous supports. An analysis of the growth behaviour of D. discoideum in the immobilised state is reported. For this purpose, D. discoideum was cultivated in continuously operated reactors in a suspension culture (homogeneous system) and immobilised on a porous support (heterogeneous system). Thus, it is possible to compare homogeneous and heterogeneous systems under steady-state conditions. Immobilisation was achieved by the colonisation of porous glass beads (SIRAN). Simple models are applied in order to describe the growth behaviour of fractions of both the cells in free suspension and the cells inside the porous carrier. This analysis shows that D. discoideum inside the pores grows at a rate of only about 10% compared with that in free solution. The consequence of this behaviour is discussed in terms of reactor performance.

摘要

社会性变形虫盘基网柄菌很适合在固定化状态下培养，最简单的方法是使其在多孔载体上定殖。本文报道了对盘基网柄菌在固定化状态下生长行为的分析。为此，将盘基网柄菌在连续运行的反应器中进行悬浮培养（均相系统），并固定在多孔载体上（非均相系统）。这样，就可以在稳态条件下比较均相和非均相系统。通过多孔玻璃珠（SIRAN）的定殖实现固定化。应用简单模型来描述游离悬浮细胞和多孔载体内细胞部分的生长行为。该分析表明，孔内的盘基网柄菌生长速率仅为游离溶液中生长速率的约10%。从反应器性能方面讨论了这种行为的后果。