Riva A, Puxeddu R, Loy F, Testa Riva F
Department of Cytomorphology, University of Cagliari, Cittadella Universitaria, Cagliari, Monserrato, Italy.
Eur J Morphol. 2002 Oct;40(4):227-33. doi: 10.1076/ejom.40.4.227.16694.
In this study, the first experimental investigation carried out at the ultrastructural level on mucous cells of human salivary glands, we have examined by light microscopy (LM), transmission electron microscopy (TEM), high resolution scanning electron microscopy (HRSEM), the secretory response of labial glands stimulated in vitro by the beta-adrenergic agent, D,L isoproterenol, and by the muscarinic agent carbachol. For comparison we have used identical methods to study samples of mixed portions of human submandibular glands. Morphological findings obtained here on both submandibular and labial glands mucous cells demonstrate that mucous droplets are released solely by muscarinic stimulation, and that cytological events occurring during secretory discharge are similar to those described by others, using TEM, on stimulated mucous cells of rat sublingual glands. Despite the fact that human labial glands are said to have a prominent cholinergic innervation with scanty adrenergic nerves, the response of seromucous cells in these organs to stimulation with carbachol and with isoproterenol was similar to that observed by us, (using LM, TEM and HRSEM), in serous cells of human major salivary glands.
在本研究中，我们首次在超微结构水平上对人唾液腺黏液细胞进行了实验研究，通过光学显微镜（LM）、透射电子显微镜（TEM）、高分辨率扫描电子显微镜（HRSEM），检测了体外经β-肾上腺素能药物D,L-异丙肾上腺素和毒蕈碱样药物卡巴胆碱刺激的唇腺的分泌反应。为作比较，我们采用相同方法研究了人下颌下腺混合部分的样本。在此获得的关于下颌下腺和唇腺黏液细胞的形态学发现表明，黏液滴仅通过毒蕈碱样刺激释放，并且分泌排出过程中发生的细胞学事件与其他人使用TEM对大鼠舌下腺受刺激黏液细胞所描述的相似。尽管据说人唇腺有明显的胆碱能神经支配且肾上腺素能神经稀少，但这些器官中的浆液黏液细胞对卡巴胆碱和异丙肾上腺素刺激的反应与我们（使用LM、TEM和HRSEM）在人主要唾液腺的浆液细胞中观察到的相似。
