针对灭活急性出血性结膜炎（AHC）病毒；肠道病毒70型（EV70）的全身和眼部抗体反应。

Systemic and ocular antibody responses to inactivated acute hemorrhagic conjunctivitis (AHC) virus; enterovirus 70 (EV70).

作者信息

Langford Marlyn P, Orillac Rogelio, Chen Dequan, Texada Donald

机构信息

Department of Ophthalmology, Louisiana State University Health Sciences Center, Shreveport, LA 70112-3932, USA.

出版信息

Ocul Immunol Inflamm. 2003 Sep;11(3):197-209. doi: 10.1076/ocii.11.3.197.17352.

DOI:10.1076/ocii.11.3.197.17352

PMID:14566646

Abstract

PURPOSE

Evaluate the immune response in rabbits injected with EV70, the agent of acute hemorrhagic conjunctivitis (AHC) and AHC associated neuropathy.

METHODS

Rabbits were injected intramuscularly with uv-light inactivated EV70 isolate J670/71. Neutralizing activity against EV70 was quantified in serum and tear samples and the immunoglobulin (Ig) classes of the neutralizing activity in serum identified by sucrose gradient ultra-centrifugation. Adjuvant muramyl dipeptide (MDP) was applied topically to assess the role of ocular inflammation on levels of neutralizing antibody, proteins and Ig in tears. The protective effects of human and rabbit sera and interferon-alpha (IFN-alpha) against EV70 were compared in human conjunctival and lens cells.

RESULTS

Sera collected at 6 and 13 d contained 19S IgM anti-EV70 neutralizing antibody, while serum collected 21 d post injection contained 19S IgM and 7S IgG anti-EV70 neutralizing antibody. Low titers of anti-EV70 activity (< or =30 U/ml) were detected in tears of seropositive rabbits. MDP induction of conjunctivitis in seropositive rabbits increased tear IgG concentration (3-fold) and anti-EV70 neutralizing antibody titers (> or =10-fold). The protective effect of the rabbit and human sera against EV70 infection in conjunctival, but not lens epithelial cells, was enhanced by the addition of IFN-alpha.

CONCLUSIONS

Immunization with uv-light inactivated EV70 elicits a classical humoral immune response in rabbits. The protective activity of serum in EV70-infected human conjunctival cells, but not lens cells, was increased by IFN-alpha. Adjuvant MDP-induced conjunctivitis, increased blood-conjunctival barrier (BCB) permeability and anti-EV70 neutralizing activity in tear of seropositive rabbits. The results suggest immunization with inactivated EV70 could provide systemic as well as ocular protection during natural EV70 infection.

摘要

目的

评估注射肠道病毒70型（EV70）的兔子的免疫反应，EV70是急性出血性结膜炎（AHC）及AHC相关神经病变的病原体。

方法

给兔子肌肉注射经紫外线灭活的EV70分离株J670/71。对血清和泪液样本中针对EV70的中和活性进行定量，并通过蔗糖梯度超速离心法确定血清中中和活性的免疫球蛋白（Ig）类别。局部应用佐剂胞壁酰二肽（MDP），以评估眼部炎症对泪液中中和抗体、蛋白质和Ig水平的作用。在人结膜细胞和晶状体细胞中比较人血清、兔血清和α干扰素（IFN-α）对EV70的保护作用。

结果

在第6天和第13天采集的血清中含有19S IgM抗EV70中和抗体，而在注射后第21天采集的血清中含有19S IgM和7S IgG抗EV70中和抗体。在血清阳性兔子的泪液中检测到低滴度的抗EV70活性（≤30 U/ml）。MDP诱导血清阳性兔子发生结膜炎，使泪液IgG浓度增加（3倍），抗EV70中和抗体滴度增加（≥10倍）。添加IFN-α可增强兔血清和人血清对结膜上皮细胞而非晶状体上皮细胞中EV70感染的保护作用。

结论

用紫外线灭活的EV70免疫可在兔子中引发经典的体液免疫反应。IFN-α可增强血清对EV70感染的人结膜细胞而非晶状体细胞的保护活性。佐剂MDP诱导的结膜炎增加了血清阳性兔子血-结膜屏障（BCB）的通透性和泪液中抗EV70的中和活性。结果表明，用灭活的EV70免疫可在自然EV70感染期间提供全身及眼部保护。