Pompe Tilo, Kobe Fritz, Salchert Katrin, Jørgensen Birgitte, Oswald Joachim, Werner Carsten
Institute of Polymer Research Dresden & The Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069 Dresden, Germany.
J Biomed Mater Res A. 2003 Nov 1;67(2):647-57. doi: 10.1002/jbm.a.10130.
Early stages of the adhesion of human endothelial cells onto a set of smooth polymer films were analyzed to reveal the modulation of cell-matrix interactions by the physicochemical constraints of predeposited fibronectin (FN). Hydrophobic and hydrophilic polymer substrates, consisting of poly(octadecene-alt-maleic anhydride) and poly(propene-alt-maleic anhydride) films, were coated with similar amounts of FN at conditions of either covalent or noncovalent immobilization. The well-defined substrates permit variation of the anchorage of FN at invariant topography, pliability, and molecular composition. Although all of the compared FN coatings were effective in stimulating attachment of endothelial cells, the initial formation of cell-matrix adhesions was found to be controlled by the type of interaction between predeposited FN and the underlying substrate. Covalent linkage and hydrophobic interactions of the predeposited FN with the polymer films interfered with the rapid generation of focal and fibrillar adhesions. It was demonstrated that this was caused by the fact that only weakly bound FN could become readily reorganized by the adherent cells. Upon prolonged culture periods at standard cell culture conditions, secretion and deposition of organized extracellular matrix by the attached cells was found to balance out the differences of the substrates.
分析了人内皮细胞在一组光滑聚合物薄膜上黏附的早期阶段，以揭示预沉积纤连蛋白（FN）的物理化学限制对细胞-基质相互作用的调节作用。由聚（十八烯-alt-马来酸酐）和聚（丙烯-alt-马来酸酐）薄膜组成的疏水和亲水聚合物基质，在共价或非共价固定条件下涂覆了相似量的FN。这些定义明确的基质允许在不变的形貌、柔韧性和分子组成下改变FN的锚定。尽管所有比较的FN涂层都能有效刺激内皮细胞的附着，但发现细胞-基质黏附的初始形成受预沉积FN与下层基质之间相互作用类型的控制。预沉积FN与聚合物薄膜的共价连接和疏水相互作用干扰了粘着斑和纤维状黏附的快速形成。结果表明，这是由于只有弱结合的FN才能被黏附细胞轻易重组。在标准细胞培养条件下延长培养时间后，发现附着细胞分泌和沉积有组织的细胞外基质能够平衡基质之间的差异。
