Morvan Daniel, Demidem Aïcha, Madelmont Jean-Claude
UMR 484, rue Montalembert, 63005 Clermont-Ferrand, France.
Pathol Biol (Paris). 2003 Jul;51(5):256-9. doi: 10.1016/s0369-8114(03)00071-3.
Phospholipid metabolism is tightly involved in tumor growth regulation and tumor cell survival. The response of phospholipid metabolism to chloroethyle nitrosourea treatment is investigated in a murine B16 melanoma model. Measurements of phospholipid derivatives are performed on intact tumor tissue samples using one- and two-dimensional proton NMR spectroscopy. During the tumor growth inhibition phase under treatment, tumors overexpress phosphocholine, phosphoethanolamine, glycerophosphocholine and glycerophosphoethanolamine, whereas phosphatidylcholine and phosphatidylethanolamine levels are maintained to control levels. During re-growth, which remained quantitatively much below control growth, chloroethyle nitrosourea-treated melanoma tumors overexpress phosphocholine and phosphoethanolamine only. In treated melanoma, phosphatidylcholine levels show an inverse relationship with tumor growth rates. In conclusion, chloroethyle nitrosourea-treated melanoma tumors maintain their phosphatidylcholine levels and exhibit transformed phospholipid metabolism phenotype, by mechanisms that could participate in tumor cell survival.
磷脂代谢与肿瘤生长调节和肿瘤细胞存活密切相关。在小鼠B16黑色素瘤模型中研究了磷脂代谢对氯乙基亚硝脲治疗的反应。使用一维和二维质子核磁共振光谱对完整肿瘤组织样本进行磷脂衍生物的测量。在治疗期间的肿瘤生长抑制阶段，肿瘤中磷酸胆碱、磷酸乙醇胺、甘油磷酸胆碱和甘油磷酸乙醇胺过表达，而磷脂酰胆碱和磷脂酰乙醇胺水平维持在对照水平。在再生长期间，其数量仍远低于对照生长，经氯乙基亚硝脲治疗的黑色素瘤肿瘤仅磷酸胆碱和磷酸乙醇胺过表达。在经治疗的黑色素瘤中，磷脂酰胆碱水平与肿瘤生长速率呈负相关。总之，经氯乙基亚硝脲治疗的黑色素瘤肿瘤维持其磷脂酰胆碱水平，并表现出转变的磷脂代谢表型，其机制可能参与肿瘤细胞存活。
