Rossi Antonietta, Serraino Ivana, Dugo Paola, Di Paola Rosanna, Mondello Luigi, Genovese Tiziana, Morabito Domenica, Dugo Giovanni, Sautebin Lidia, Caputi Achille P, Cuzzocrea Salvatore
Dipartimento di Farmacologia Sperimentale, Università "Federico II", Via D. Montesano 49, 80131 Napoli, Italy.
Free Radic Res. 2003 Aug;37(8):891-900. doi: 10.1080/1071576031000112690.
Anthocyanins are a group of naturally occuring phenolic compounds related to the coloring of plants, flowers and fruits. These pigments are important as quality indicators, as chemotaxonomic markers and for their antioxidant activities. Here, we have investigated the therapeutic efficacy of anthocyanins contained in blackberry extract (cyanidin-3-O-glucoside represents about 80% of the total anthocyanin contents) in an experimental model of lung inflammation induced by carrageenan in rats. Injection of carrageenan into the pleural cavity elicited an acute inflammatory response characterized by fluid accumulation which contained a large number of neutrophils as well as an infiltration of polymorphonuclear leukocytes in lung tissues and subsequent lipid peroxidation, and increased production of nitrite/nitrate (NOx) and prostaglandin E2 (PGE2). All parameters of inflammation were attenuated in a dose-dependent manner by anthocyanins (10, 30 mg kg(-1) 30 min before carrageenan). Furthermore, carrageenan induced an upregulation of the adhesion molecule ICAM-1, nitrotyrosine and poly (ADP-ribose) synthetase (PARS) as determined by immunohistochemical analysis of lung tissues. The degree of staining was lowered by anthocyanins treatment. Thus, the anthocyanins contained in the blackberry extract exert multiple protective effects in carrageenan-induced pleurisy.
花青素是一类与植物、花朵和果实颜色相关的天然酚类化合物。这些色素作为质量指标、化学分类标记以及因其抗氧化活性而具有重要意义。在此，我们研究了黑莓提取物中所含花青素（矢车菊素 - 3 - O - 葡萄糖苷约占总花青素含量的80%）在角叉菜胶诱导的大鼠肺部炎症实验模型中的治疗效果。向胸腔注射角叉菜胶引发了急性炎症反应，其特征为液体蓄积，其中含有大量中性粒细胞，以及肺组织中多形核白细胞浸润和随后的脂质过氧化，同时亚硝酸盐/硝酸盐（NOx）和前列腺素E2（PGE2）的产生增加。花青素（在注射角叉菜胶前30分钟给予10、30 mg kg⁻¹）以剂量依赖的方式减轻了所有炎症参数。此外，通过对肺组织的免疫组织化学分析确定，角叉菜胶诱导了黏附分子ICAM - 1、硝基酪氨酸和聚（ADP - 核糖）合成酶（PARS）的上调。花青素处理降低了染色程度。因此，黑莓提取物中所含的花青素在角叉菜胶诱导的胸膜炎中发挥多种保护作用。
