Mihaylova Dasha, Desseva Ivelina, Tumbarski Yulian, Popova Aneta, Pandova Svetla, Lante Anna
Department of Biotechnology, University of Food Technologies, 4002 Plovdiv, Bulgaria.
Department of Analytical Chemistry and Physical Chemistry, University of Food Technologies, 4002 Plovdiv, Bulgaria.
Plants (Basel). 2024 Oct 20;13(20):2936. doi: 10.3390/plants13202936.
The current study focuses on evaluating the enzyme inhibition (acetylcholinesterase, α-amylase, α-glucosidase, pancreatin lipase), antioxidant, and antimicrobial activities of the "Modesto" apricot, "Stanley" plum, and their hybrid the "Stendesto" plum-apricot. The "Stendesto" is the only successful plum-apricot hybrid in Bulgaria. A spectrophotometric approach was used to evaluate the antioxidant activity following four complementary assays (DPPH, ABTS, FRAP, and CUPRAC). The "Stendesto" plum-apricot revealed its enhanced antioxidant potential compared to its parental lines. Apart from the free phenolics extraction, two other techniques (alkaline and acid hydrolysis) were applied to reveal the biological potential of the studied fruit. Not only free but also bound phenolic extracts were able to inhibit α-glucosidase and acetylcholinesterase, while none of the extracts inhibited lipase or α-amylase. None of the apricot extracts had antimicrobial activity, while the other fruit had limited antimicrobial activity. The proposed results undoubtedly reveal that hybrid fruits possess enhanced biological activity compared to their parents. This is a first comprehensive evaluation of hybrid fruits with reference to parental lines. This makes them an interesting research topic that should be better explored.
当前研究聚焦于评估“莫德斯托”杏、“斯坦利”李及其杂交品种“斯滕德斯托”李杏的酶抑制活性（乙酰胆碱酯酶、α-淀粉酶、α-葡萄糖苷酶、胰脂肪酶）、抗氧化活性和抗菌活性。“斯滕德斯托”是保加利亚唯一成功的李杏杂交品种。采用分光光度法，通过四种互补测定法（DPPH、ABTS、FRAP和CUPRAC）评估抗氧化活性。与亲本相比，“斯滕德斯托”李杏展现出更强的抗氧化潜力。除了游离酚类物质提取外，还应用了另外两种技术（碱水解和酸水解）来揭示所研究水果的生物活性。不仅游离酚类提取物，而且结合酚类提取物都能够抑制α-葡萄糖苷酶和乙酰胆碱酯酶，而所有提取物均未抑制脂肪酶或α-淀粉酶。杏提取物均无抗菌活性，而其他水果的抗菌活性有限。所提出的结果无疑表明，杂交果实与其亲本相比具有更强的生物活性。这是首次参照亲本对杂交果实进行全面评估。这使得它们成为一个值得深入探索的有趣研究课题。
