核磁共振中用于研究大型生物大分子结构与功能的横向弛豫优化谱（TROSY）

TROSY in NMR studies of the structure and function of large biological macromolecules.

作者信息

Fernández César, Wider Gerhard

机构信息

Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule Zürich, CH-8093, Zürich, Switzerland.

出版信息

Curr Opin Struct Biol. 2003 Oct;13(5):570-80. doi: 10.1016/j.sbi.2003.09.009.

DOI:10.1016/j.sbi.2003.09.009

PMID:14568611

Abstract

Transverse relaxation-optimized spectroscopy (TROSY), in combination with various isotope-labeling techniques, has opened avenues to study biomolecules with molecular masses of up to 1000000Da by solution NMR. Important recent applications of TROSY include the structure determination of membrane proteins in detergent micelles, structural and functional studies of large proteins in both monomeric form and macromolecular complexes, and investigations of intermolecular interactions in large complexes. TROSY improves the measurement of residual dipolar couplings and the detection of scalar couplings across hydrogen bonds - techniques that promise to further enhance the determination of solution structures of large proteins and oligonucleotides.

摘要

横向弛豫优化光谱法（TROSY）与各种同位素标记技术相结合，为通过溶液核磁共振研究分子量高达1000000Da的生物分子开辟了道路。TROSY最近的重要应用包括去污剂胶束中膜蛋白的结构测定、单体形式和大分子复合物中大型蛋白质的结构与功能研究，以及大型复合物中分子间相互作用的研究。TROSY改进了残余偶极耦合的测量以及跨氢键标量耦合的检测——这些技术有望进一步提高大型蛋白质和寡核苷酸溶液结构的测定。