Riek R, Pervushin K, Wüthrich K
Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule Hönggerberg, CH-8093, Zürich, Switzerland.
Trends Biochem Sci. 2000 Oct;25(10):462-8. doi: 10.1016/s0968-0004(00)01665-0.
TROSY and CRINEPT are new techniques for solution NMR studies of molecular and supramolecular structures. They allow the collection of high-resolution spectra of structures with molecular weights >100 kDa, significantly extending the range of macromolecular systems that can be studied by NMR in solution. TROSY has already been used to map protein-protein interfaces, to conduct structural studies on membrane proteins and to study nucleic acid conformations in multimolecular assemblies. These techniques will help us to investigate the conformational states of individual macromolecular components and will support de novo protein structure determination in large supramolecular structures.
TROSY和CRINEPT是用于溶液核磁共振研究分子和超分子结构的新技术。它们能够收集分子量大于100 kDa的结构的高分辨率光谱，显著扩展了可通过溶液核磁共振研究的大分子系统的范围。TROSY已被用于绘制蛋白质-蛋白质界面、进行膜蛋白的结构研究以及研究多分子组装体中的核酸构象。这些技术将帮助我们研究单个大分子组分的构象状态，并支持在大型超分子结构中从头确定蛋白质结构。
