Lu Y, Loh P C
Department of Microbiology, University of Hawaii, Honolulu.
Arch Virol. 1992;127(1-4):339-43. doi: 10.1007/BF01309596.
Some of the relevant biological properties of a rhabdovirus isolated from penaeid shrimps (RPS) were examined. The virus replicated in an established fish cell line, epithelioma papulosum cyprini (EPC) which allowed for the development of a quantitative plaque assay protocol. Virus replication was not inhibited by the DNA antagonist, 5-bromo-2'-deoxyuridine (20 micrograms/ml). Virus infectivity was sensitive to 20% ethyl ether, low pH, and to 37 degrees C. The virus showed marked lability to repeated freezing and thawing and storage at -10 degrees C, but was stable at -70 degrees C for several weeks. The virus particle to infectious unit ratio in EPC was found to be 30.
对从对虾中分离出的一种弹状病毒（RPS）的一些相关生物学特性进行了检测。该病毒能在已建立的鱼类细胞系——鲤上皮瘤细胞（EPC）中复制，这使得能够开发出一种定量蚀斑测定方案。病毒复制不受DNA拮抗剂5-溴-2'-脱氧尿苷（20微克/毫升）的抑制。病毒感染性对20%乙醚、低pH值以及37摄氏度敏感。该病毒对反复冻融和在-10摄氏度下储存表现出明显的不稳定性，但在-70摄氏度下可稳定保存数周。在EPC中发现病毒粒子与感染单位的比率为30。
