Balci Veysel, Ozgüç Halil, Akköse Sule, Kahveci Nevzat, Serdar Zehra, Tokyay Rifat
Uludag University, Faculty of Medicine, Department of Emergency Medicine, Bursa, Turkey.
Ulus Travma Acil Cerrahi Derg. 2003 Oct;9(4):239-45.
In an experimental model of crush injury, tissue blood flow, the extend of oxidant injury and the effect of lactated ringer's resuscitation were investigated.
Rats were divided into sham (n: 8), crush injury (n: 8), and crush injury + lactated ringer's resuscitation (n: 8) groups. Arterial and venous catheterization were performed in all groups. Crush injury was done with intravenous infusion of allogenic muscle extract. In the crush injury + lactated ringer's resuscitation group 30 ml/kg lactated ringer's solution was infused in 30 minutes. Kidney and liver blood flow were measured by using a laser flowmeter. To assess the oxidant injury blood, liver, and kidney tissue samples were collected for malondialdehyde and glutathione measurements.
In the crush injury, diminished liver and kidney blood flow rates were improved with lactated ringer's resuscitation. In addition, glutathione levels decreased whereas malondialdehyde levels and base deficit increased. Lactated ringer's resuscitation brought base deficit to the control levels. When compared with the crush injury, lactated ringer's infusion increased the glutathione levels but could not decrease the malondialdehyde ones.
Lactated ringer's resuscitation improved the blood flow rates but could not prevent oxidant injury totally.
在挤压伤实验模型中，研究了组织血流、氧化损伤程度以及乳酸林格氏液复苏的效果。
将大鼠分为假手术组（n = 8）、挤压伤组（n = 8）和挤压伤+乳酸林格氏液复苏组（n = 8）。所有组均进行动脉和静脉插管。通过静脉输注同种异体肌肉提取物造成挤压伤。在挤压伤+乳酸林格氏液复苏组中，在30分钟内输注30 ml/kg乳酸林格氏液。使用激光流量计测量肾脏和肝脏血流。为评估氧化损伤，采集血液、肝脏和肾脏组织样本以测量丙二醛和谷胱甘肽。
在挤压伤组中，乳酸林格氏液复苏改善了肝脏和肾脏血流速率的降低。此外，谷胱甘肽水平降低，而丙二醛水平和碱缺失增加。乳酸林格氏液复苏使碱缺失恢复到对照水平。与挤压伤组相比，输注乳酸林格氏液增加了谷胱甘肽水平，但未能降低丙二醛水平。
乳酸林格氏液复苏改善了血流速率，但不能完全预防氧化损伤。
