Gai Yun, Gao Rui-lan, Niu Yang-ping
Affiliated Hospital, Zhejiang TCM College, Hangzhou 310006.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2003 Sep;23(9):680-3.
To study the effect of panax notoginsenosides (PNS) on the proliferation of hematopoietic progenitor cells (HPC) in mice with immune-mediated aplastic anemia.
Balb/c mice model of immune-mediated aplastic anemia was established by radiation with sublethal dose of 60Co following the intravenously infusing lymphocytes of DBA/2 mice. Model mice in the treated groups were treated separately with high, middle and low dose of PNS, 3.2 mg, 1.6 mg and 0.8 mg per day respectively by intraperitoneal injection. Model mice in the control group and normal mice in the normal control group were treated with normal saline. The peripheral white blood cell (WBC) count and pathological examination of bone marrow were carried out 12 days later, the bone marrow was taken to be incubated in semi-solid culture system for observing proliferation of HPC.
PNS could (1) increase peripheral WBC count: as compared with that in the model control, WBC in the high, middle and low dose PNS groups was raised by (34.3 +/- 2.9)%, (29.2 +/- 1.7)% and (14.5 +/- 1.6)% respectively, P < 0.01 and P < 0.05; (2) improve the bone marrow inhibition: pathological examination showed in the model group, the hematopoietic structure was destroyed and replaced by fatty tissue, while in the PNS treated groups, the structure of marrow was rather complete and filled with abundant hematopoietic cells; (3) promote the proliferation of HPC: as compared with the model group, the colony formation of CFU-GM were increased by (64.4 +/- 2.8)%, (67.3 +/- 2.4)% and (21.9 +/- 1.8)% respectively and that of CFU-E increased by (31.9 +/- 3.6)%, (20.7 +/- 2.4)% and (12.8 +/- 2.6)% respectively in the three PNS treated group (P < 0.01 and P < 0.05).
PNS could enhance hematopoiesis by promoting proliferation of CFU-GM and CFU-E progenitors so as to improve the hematopoietic function in mice of immune-mediated aplastic anemia.
研究三七总皂苷（PNS）对免疫介导的再生障碍性贫血小鼠造血祖细胞（HPC）增殖的影响。
采用60Co亚致死剂量照射Balb/c小鼠后，静脉输注DBA/2小鼠淋巴细胞，建立免疫介导的再生障碍性贫血小鼠模型。治疗组模型小鼠分别腹腔注射高、中、低剂量PNS，分别为每天3.2 mg、1.6 mg和0.8 mg。对照组模型小鼠和正常对照组正常小鼠均给予生理盐水。12天后进行外周血白细胞（WBC）计数及骨髓病理检查，取骨髓在半固体培养体系中培养，观察HPC增殖情况。
PNS可（1）增加外周血白细胞计数：与模型对照组相比，高、中、低剂量PNS组白细胞分别升高（34.3±2.9）%、（29.2±1.7）%和（14.5±1.6）%，P<0.01及P<0.05；（2）改善骨髓抑制：病理检查显示模型组造血结构破坏，被脂肪组织替代，而PNS治疗组骨髓结构较完整，充满丰富的造血细胞；（3）促进HPC增殖：与模型组相比，三个PNS治疗组CFU-GM集落形成分别增加（64.4±2.8）%、（67.3±2.4）%和（21.9±1.8）%，CFU-E集落形成分别增加（31.9±3.6）%、（20.7±2.4）%和（12.8±2.6）%（P<0.01及P<0.05）。
PNS可通过促进CFU-GM和CFU-E祖细胞增殖增强造血功能，从而改善免疫介导的再生障碍性贫血小鼠的造血功能。
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