Zhou Jian, Wu Rong, Wang Long
Department of Cardiology, Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730000, China. *Corresponding author, E-mail:
Department of Cardiology, Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730000, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2018 Jul;34(7):605-611.
Objective To observe the effect of panax notoginsenosides (PNS) on cardiac function of rats after acute myocardial infarction (AMI) and investigate the influence of PNS on the mobilization of bone marrow-derived mesenchymal stem cells (BM-MSCs). Methods A total of 48 rats were randomly assigned into the sham group, AMI group, low-dose PNS group [100 mg/(kg.d)] and high-dose PNS group [500 mg/(kg.d)]. The rat model of AMI was established by coronary ligation, and 6 rats were sacrificed in each group after 7 and 21 days of treatment with high and low doses of PNS. The heart function of rats was detected by echocardiography before execution, and peripheral blood and heart tissue were collected. Flow cytometry was used to test the proportions of CD90, CD105, CD54 or CD106 positive cells in the peripheral blood. ELISA was performed to measure the levels of stem cell factor (SCF) in the peripheral blood. TTC staining was applied to evaluate the infarct size of myocardial tissues. TUNEL assay was carried out to determine the apoptosis of myocardial tissues and immunohistochemistry to determine the expression levels of CD105 in the infarction area. Results Compared with the sham group, the percentage of apoptotic cells in the AMI group significantly increased. Seven days or 21 days after the intervention with PNS, the infarction area and the apoptotic rates in the PNS treated groups were observably alleviated when compared with the AMI group. In addition, at 7 days and 21 days after operation, the LVEF and LVFS decreased, whereas the LVIDs, LVIDd, LVEDV and LVESV significantly decreased in the AMI groups when compared with the sham group. Treatment with PNS could effectively improve the above alterations. When compared with the AMI group, PNS treatment significantly increased the proportions of CD90 or CD105 positive cells and the concentration of SCF, whereas decrease the proportions of CD54 or CD106 positive cells in the peripheral blood in a dose-dependent manner. Moreover, the level of CD105 in the marginal zone of AMI was significant higher in the PNS treated groups when compared with that in the AMI group. Conclusion PNS treatment improves left ventricular function after AMI, which may be related to PNS inhibiting the apoptosis of myocardocytes and promoting the mobilization of BM-MSCs.
目的 观察三七总皂苷（PNS）对急性心肌梗死（AMI）大鼠心功能的影响，并探讨PNS对骨髓间充质干细胞（BM-MSCs）动员的影响。方法 将48只大鼠随机分为假手术组、AMI组、低剂量PNS组[100 mg/(kg·d)]和高剂量PNS组[500 mg/(kg·d)]。采用冠状动脉结扎法建立AMI大鼠模型，高低剂量PNS治疗7天和21天后，每组处死6只大鼠。处死前通过超声心动图检测大鼠心功能，并采集外周血和心脏组织。采用流式细胞术检测外周血中CD90、CD105、CD54或CD106阳性细胞的比例。采用酶联免疫吸附测定法（ELISA）检测外周血中干细胞因子（SCF）水平。采用TTC染色评估心肌组织梗死面积。采用TUNEL法检测心肌组织凋亡情况，采用免疫组织化学法检测梗死区CD105表达水平。结果 与假手术组相比，AMI组凋亡细胞百分比显著增加。PNS干预7天或21天后，与AMI组相比，PNS治疗组梗死面积和凋亡率明显减轻。此外，与假手术组相比，AMI组术后7天和21天左室射血分数（LVEF）和左室短轴缩短率（LVFS）降低，而左室舒张末期内径（LVIDs）、左室舒张末期内径（LVIDd）、左室舒张末期容积（LVEDV）和左室收缩末期容积（LVESV）显著降低。PNS治疗可有效改善上述改变。与AMI组相比，PNS治疗可显著增加外周血中CD90或CD105阳性细胞比例和SCF浓度，同时呈剂量依赖性降低外周血中CD54或CD106阳性细胞比例。此外，与AMI组相比，PNS治疗组AMI边缘区CD105水平显著升高。结论 PNS治疗可改善AMI后左心室功能，这可能与PNS抑制心肌细胞凋亡和促进BM-MSCs动员有关。
