Lee Sanghoo, Seo Dong-Hyuk, Park Hey-Lin, Choi Youngjin, Jung Seunho
Department of Microbial Engineering and Bio/Molecular Informatics Center, Konkuk University, 1 Hwayang-dong,Gwangjin-gu, Seoul 143- 701, South Korea.
Antonie Van Leeuwenhoek. 2003;84(3):201-7. doi: 10.1023/a:1026075215921.
A plant flavone, luteolin is a well-known inducer of nod genes in the Rhizobium meliloti. Its poor aqueous solubility was greatly enhanced by the complexation with a family of cyclosophoraoses synthesized in R.meliloti. Nuclear magnetic resonance (NMR) spectroscopic analysis showed that the chemical shifts of the aromatic ring moieties of the luteolin were changed greatly by the complexation with cyclosophoraoses. Fourier transform infrared (FTIR) spectroscopic analysis also showed a restricted vibrational pattern in carbonyl stretching region of the luteolin due to the complexation. This effective complex formation of cyclosophoraoses with a plant flavone, luteolin, suggests that rhizobial cyclosophoraoses play an important role as a solubility enhancer of the hydrophobic legume-derived flavonoids.
植物黄酮木犀草素是已知的苜蓿根瘤菌中结瘤基因的诱导剂。通过与苜蓿根瘤菌中合成的一类环槐糖进行络合，其较差的水溶性得到了极大提高。核磁共振（NMR）光谱分析表明，木犀草素芳香环部分的化学位移因与环槐糖络合而发生了很大变化。傅里叶变换红外（FTIR）光谱分析也表明，由于络合作用，木犀草素羰基伸缩区域的振动模式受到限制。环槐糖与植物黄酮木犀草素的这种有效络合表明，根瘤菌环槐糖作为疏水性豆科植物来源黄酮类化合物的溶解度增强剂发挥着重要作用。
