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胺化酵母聚糖的合成、表征及生物活性

Synthesis, Characterization, and Biological Activity of Aminated Zymosan.

作者信息

Venkatachalam Geetha, Arumugam Senthilkumar, Doble Mukesh

机构信息

Bioengineering and Drug Design Lab, Department of Biotechnology, IIT-Madras, Chennai 600036, India.

出版信息

ACS Omega. 2020 Jun 23;5(26):15973-15982. doi: 10.1021/acsomega.0c01243. eCollection 2020 Jul 7.

DOI:10.1021/acsomega.0c01243
PMID:32656418
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7345428/
Abstract

Zymosan (ZM), a naturally occurring insoluble macromolecule obtained from the cell wall of , is used as a functional food (as dietary fiber), phagocytic stimulus, and immune potentiator. The present study aimed to increase its solubility and evaluate its immunological application. ZM was converted into soluble 6-amino-6-deoxy-β-(1-3)-glucan of a molecular weight of 296 kDa by reduction. Detailed structural characterization of aminated ZM was determined by Fourier transform infrared spectroscopy and two-dimensional NMR analysis (2D, COSY, TOCSY, ROSEY, NOSEY, and HSQC). Aminated ZM was biocompatible with Raw 264.7 macrophage cell lines up to a concentration of 100 μg/mL. Rhodamine tagging revealed that the aminated ZM microparticles were found localized within the nucleus of Raw 264.7 cells. Both native and aminated ZM showed a similar expression pattern of inflammatory genes in Raw 264.7.

摘要

酵母聚糖（ZM）是一种从[具体来源]细胞壁获得的天然存在的不溶性大分子，用作功能性食品（作为膳食纤维）、吞噬刺激剂和免疫增强剂。本研究旨在提高其溶解度并评估其免疫学应用。通过还原反应，ZM被转化为分子量为296 kDa的可溶性6-氨基-6-脱氧-β-(1-3)-葡聚糖。通过傅里叶变换红外光谱和二维核磁共振分析（二维、COSY、TOCSY、ROSEY、NOSEY和HSQC）确定了胺化ZM的详细结构特征。胺化ZM在浓度高达100μg/mL时与Raw 264.7巨噬细胞系具有生物相容性。罗丹明标记显示胺化ZM微粒定位于Raw 264.7细胞的细胞核内。天然ZM和胺化ZM在Raw 264.7中均显示出相似的炎症基因表达模式。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca51/7345428/1ac5f816441e/ao0c01243_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca51/7345428/975a47f6735e/ao0c01243_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca51/7345428/eaa7dd6171fa/ao0c01243_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca51/7345428/28ec1a615aa7/ao0c01243_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca51/7345428/f389e18ba2a3/ao0c01243_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca51/7345428/f93f5415e442/ao0c01243_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca51/7345428/1ac5f816441e/ao0c01243_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca51/7345428/975a47f6735e/ao0c01243_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca51/7345428/eaa7dd6171fa/ao0c01243_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca51/7345428/28ec1a615aa7/ao0c01243_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca51/7345428/f389e18ba2a3/ao0c01243_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca51/7345428/f93f5415e442/ao0c01243_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca51/7345428/1ac5f816441e/ao0c01243_0006.jpg

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