Paz-Silva A, Sánchez-Andrade R, Suárez J L, Pedreira J, Arias M, López C, Panadero R, Díaz P, Díez-Baños P, Morrondo P
Departamento de Patología Animal Parasitología y Enfermedades Parasitarias, Facultad de Veterinaria, Universidad de Santiago de Compostela, 27071, Lugo, Spain.
Parasitol Res. 2003 Oct;91(4):328-31. doi: 10.1007/s00436-003-0961-z. Epub 2003 Sep 9.
The prevalence of fasciolosis in sheep (Galicia, Northwest Spain) kept under field conditions was determined by using a sandwich-enzyme-linked immunosorbent assay (sELISA). Serum Fasciola hepatica circulating antigens were captured by means of a rabbit polyclonal IgG antibody to F. hepatica excretory/secretory products. Results were compared to those obtained by faecal sedimentation and an indirect ELISA (iELISA) and excretory/secretory antigens. Prevalences were 39.1% by sELISA, 30.4% by faecal sedimentation and 56% by iELISA; 83.3% of the sheep were positive to any one of the three tests. We observed that 59.5% of the sheep examined had active fasciolosis, 29.1% (117) had antigenaemia, 20.4% (82) passed eggs, and 40 (10%) were positive to both probes. We conclude that there is a high prevalence of fasciolosis in sheep from the studied region, and that the combination of sELISA and coprological sedimentation is extremely helpful for demonstrating current fasciolosis, so its application can be strongly recommended for epidemiological surveys.
采用夹心酶联免疫吸附测定法（sELISA）测定了西班牙西北部加利西亚地区野外饲养绵羊的肝片吸虫病患病率。利用兔抗肝片吸虫排泄/分泌产物多克隆IgG抗体捕获血清中肝片吸虫循环抗原。将结果与粪便沉淀法、间接酶联免疫吸附测定法（iELISA）以及排泄/分泌抗原检测结果进行比较。sELISA法测得的患病率为39.1%，粪便沉淀法为30.4%，iELISA法为56%；83.3%的绵羊对这三种检测方法中的任何一种呈阳性反应。我们观察到，所检测的绵羊中有59.5%患有活动性肝片吸虫病，29.1%（117只）有抗原血症，20.4%（82只）排出虫卵，40只（10%）对两种检测均呈阳性。我们得出结论，研究区域内绵羊的肝片吸虫病患病率很高，并且sELISA法与粪便学沉淀法相结合对于诊断当前的肝片吸虫病非常有帮助，因此强烈推荐将其应用于流行病学调查。
