从重组黄色粘球菌发酵液中分离和鉴定新型埃坡霉素类似物

Isolation and characterization of new epothilone analogues from recombinant Myxococcus xanthus fermentations.

作者信息

Starks Courtney M, Zhou Yiqing, Liu Fenghua, Licari Peter J

机构信息

Kosan Biosciences, Inc., 3832 Bay Center Place, Hayward, California 94545, USA.

出版信息

J Nat Prod. 2003 Oct;66(10):1313-7. doi: 10.1021/np030218+.

DOI:10.1021/np030218+

PMID:14575429

Abstract

Nine new epothilone analogues (6-8, 10, 11a, 11b, 12, 13, and 15) were isolated from fermentations of Myxococcus xanthus strains engineered with modified polyketide synthase genes. The epothilone structures were elucidated primarily through interpretation of 1D and 2D NMR data. 4-Desmethyl-10,11-didehydroepothilone D (6) displayed activity against several tumor cell lines, including a multi-drug-resistant cell line.

摘要

从用修饰的聚酮合酶基因工程改造的黄色粘球菌菌株发酵产物中分离出9种新的埃坡霉素类似物（6 - 8、10、11a、11b、12、13和15）。埃坡霉素的结构主要通过对一维和二维核磁共振数据的解读来阐明。4 - 去甲基 - 10,11 - 二脱氢埃坡霉素D（6）对几种肿瘤细胞系显示出活性，包括一种多药耐药细胞系。