Goltsev V, Zaharieva I, Lambrev P, Yordanov I, Strasser R
Department of Biophysics and Radiobiology, Faculty of Biology, St. Kliment Ohridski University of Sofia, 8, Dragan Tzankov Blvd., 1164, Sofia, Bulgaria.
J Theor Biol. 2003 Nov 21;225(2):171-83. doi: 10.1016/s0022-5193(03)00236-4.
An attempt is made to reveal the relation between the induction curves of delayed fluorescence (DF) registered at 0.35-5.5 ms and the prompt chlorophyll fluorescence (PF). A simple formulation was proposed to link the ratio of the transient values of delayed and variable fluorescence with the redox state of the primary electron acceptor of Photosystem II--QA, and the thylakoid membrane energization. The term luminescence potential (UL) was introduced, defined as the sum of the redox potential of QA and the transmembrane proton gradient. It was shown that UL is proportional to the ratio of DF to the variable part of PF. The theoretical model was verified and demonstrated by analysing induction courses of PF and millisecond DF, simultaneously registered from leaves of barley--wild-type and the chlorophyll b-less mutant chlorina f2. A definitive correlation between PF and DF was established. If the luminescence changes are strictly due to UL, the courses of DF and PF are reciprocal and the millisecond DF curve resembles the first derivative of the PFt function.
人们试图揭示在0.35 - 5.5毫秒记录的延迟荧光（DF）诱导曲线与即时叶绿素荧光（PF）之间的关系。提出了一种简单的公式，将延迟荧光和可变荧光的瞬态值之比与光系统II初级电子受体QA的氧化还原状态以及类囊体膜的能量化联系起来。引入了发光电位（UL）这一术语，定义为QA的氧化还原电位与跨膜质子梯度之和。结果表明，UL与DF和PF可变部分的比值成正比。通过分析从大麦野生型和叶绿素b缺失突变体chlorina f2叶片同时记录的PF和毫秒DF的诱导过程，对该理论模型进行了验证和论证。建立了PF和DF之间的确切相关性。如果发光变化严格归因于UL，那么DF和PF的过程是相互的，并且毫秒DF曲线类似于PFt函数的一阶导数。
