Rüter Jens, Kobelt Peter, Tebbe Johannes J, Avsar Yeşim, Veh Rüdiger, Wang Lixin, Klapp Burghard F, Wiedenmann Bertram, Taché Yvette, Mönnikes Hubert
Department of Medicine, Charité, Campus Mitte, Humboldt-Universität, Berlin, Germany.
Brain Res. 2003 Nov 21;991(1-2):26-33. doi: 10.1016/j.brainres.2003.07.005.
Ghrelin is a 28-amino acid peptide hormone secreted from the stomach that acts as a gut-brain peptide with potent stimulatory effects on food intake. The aim of the present study was to investigate the effects of peripheral ghrelin (1 and 10 nmol/rat) injected intraperitoneally (i.p.) on food intake and neuronal activity in the hypothalamus and brain stem, as assessed by c-Fos-like-immunoreactivity (c-FLI), using a confocal laser scanning microscope (cLSM) as a sensitive microscopic technique to detect c-FLI-positive neurons. Cumulative food intake was significantly increased 5.3- and 3.7-fold for the 4-h period after i.p. injection of ghrelin at both doses. The number of c-FLI-positive neurons in the paraventricular nucleus of the hypothalamus (PVN) was significantly increased after peripheral administration of ghrelin (1 nmol i.p.; median: 41.8) compared with i.p. saline (median: 17.5). As described before, c-fos expression was increased in the arcuate nucleus of the hypothalamus (ARC). In the nucleus of the solitary tract (NTS) or the area postrema (AP), there was no significant change in the density of c-FLI-positive neurons. Our data suggest that an activation of the arcuate-paraventricular axis may be part of the brain circuits involved in the orexigenic effect of peripheral ghrelin.
胃饥饿素是一种由胃分泌的含28个氨基酸的肽类激素，作为一种肠脑肽，对食物摄入具有强大的刺激作用。本研究的目的是通过c-Fos样免疫反应性（c-FLI）评估腹腔注射外周胃饥饿素（1和10 nmol/大鼠）对下丘脑和脑干食物摄入及神经元活动的影响，使用共聚焦激光扫描显微镜（cLSM）作为检测c-FLI阳性神经元的灵敏显微技术。腹腔注射两种剂量的胃饥饿素后4小时内，累积食物摄入量显著增加，分别增加了5.3倍和3.7倍。与腹腔注射生理盐水（中位数：17.5）相比，外周给予胃饥饿素（腹腔注射1 nmol；中位数：41.8）后，下丘脑室旁核（PVN）中c-FLI阳性神经元数量显著增加。如前所述，下丘脑弓状核（ARC）中c-fos表达增加。在孤束核（NTS）或最后区（AP），c-FLI阳性神经元密度无显著变化。我们的数据表明，弓状核-室旁核轴的激活可能是外周胃饥饿素促食欲作用所涉及的脑回路的一部分。
